Measuring subcellular cAMP levels using FRET-based biosensing and confocal microscopy

Debasmita Banik; Paolo Annibale

doi:10.1364/FIO.2024.JW5A.33

Measuring subcellular cAMP levels using FRET-based biosensing and confocal microscopy

Debasmita Banik, Paolo Annibale^*

^*Corresponding author for this work

School of Physics and Astronomy

Research output: Contribution to conference › Paper › peer-review

Abstract

Accurate measurement of second messenger levels underpins our ability to model subcellular compartments. We combine confocal-based sensitized emission fluorescence resonance energy transfer (FRET) to tailored data analysis to extract accurate concentrations of nuclear cAMP.

Original language	English
DOIs	https://doi.org/10.1364/FIO.2024.JW5A.33
Publication status	Published - Nov 2024
Event	Frontiers in Optics + Laser Science 2024 - Colorado Convention Center, Denver, United States Duration: 23 Sept 2024 → 26 Sept 2024

Conference

Conference	Frontiers in Optics + Laser Science 2024
Abbreviated title	FiOLS 2024
Country/Territory	United States
City	Denver
Period	23/09/24 → 26/09/24

Access to Document

10.1364/FIO.2024.JW5A.33

Cite this

@conference{23e323edd6e9488c990de84c6c928b50,

title = "Measuring subcellular cAMP levels using FRET-based biosensing and confocal microscopy",

abstract = "Accurate measurement of second messenger levels underpins our ability to model subcellular compartments. We combine confocal-based sensitized emission fluorescence resonance energy transfer (FRET) to tailored data analysis to extract accurate concentrations of nuclear cAMP.",

author = "Debasmita Banik and Paolo Annibale",

year = "2024",

month = nov,

doi = "10.1364/FIO.2024.JW5A.33",

language = "English",

note = "Frontiers in Optics + Laser Science 2024, FiOLS 2024 ; Conference date: 23-09-2024 Through 26-09-2024",

}

TY - CONF

T1 - Measuring subcellular cAMP levels using FRET-based biosensing and confocal microscopy

AU - Banik, Debasmita

AU - Annibale, Paolo

PY - 2024/11

Y1 - 2024/11

N2 - Accurate measurement of second messenger levels underpins our ability to model subcellular compartments. We combine confocal-based sensitized emission fluorescence resonance energy transfer (FRET) to tailored data analysis to extract accurate concentrations of nuclear cAMP.

AB - Accurate measurement of second messenger levels underpins our ability to model subcellular compartments. We combine confocal-based sensitized emission fluorescence resonance energy transfer (FRET) to tailored data analysis to extract accurate concentrations of nuclear cAMP.

U2 - 10.1364/FIO.2024.JW5A.33

DO - 10.1364/FIO.2024.JW5A.33

M3 - Paper

AN - SCOPUS:85216028395

T2 - Frontiers in Optics + Laser Science 2024

Y2 - 23 September 2024 through 26 September 2024

ER -

Measuring subcellular cAMP levels using FRET-based biosensing and confocal microscopy

Abstract

Conference

Access to Document

Fingerprint

Cite this