Measuring subcellular cAMP levels using FRET-based biosensing and confocal microscopy

Debasmita Banik; Paolo Annibale

Measuring subcellular cAMP levels using FRET-based biosensing and confocal microscopy

Debasmita Banik, Paolo Annibale^*

^*Corresponding author for this work

School of Physics and Astronomy

Research output: Contribution to conference › Paper › peer-review

Abstract

Accurate measurement of second messenger levels underpins our ability to model subcellular compartments. We combine confocal-based sensitized emission fluorescence resonance energy transfer (FRET) to tailored data analysis to extract accurate concentrations of nuclear cAMP.

Original language	English
Publication status	Published - 2024
Event	2024 Laser Science, LS 2024 - Denver, United States Duration: 23 Sept 2024 → 26 Sept 2024

Conference

Conference	2024 Laser Science, LS 2024
Country/Territory	United States
City	Denver
Period	23/09/24 → 26/09/24

Cite this

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title = "Measuring subcellular cAMP levels using FRET-based biosensing and confocal microscopy",

abstract = "Accurate measurement of second messenger levels underpins our ability to model subcellular compartments. We combine confocal-based sensitized emission fluorescence resonance energy transfer (FRET) to tailored data analysis to extract accurate concentrations of nuclear cAMP.",

author = "Debasmita Banik and Paolo Annibale",

note = "Publisher Copyright: {\textcopyright} 2024 The Author(s). {\textcopyright} Optica Publishing Group 2024.; 2024 Laser Science, LS 2024 ; Conference date: 23-09-2024 Through 26-09-2024",

year = "2024",

language = "English",

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TY - CONF

T1 - Measuring subcellular cAMP levels using FRET-based biosensing and confocal microscopy

AU - Banik, Debasmita

AU - Annibale, Paolo

PY - 2024

Y1 - 2024

N2 - Accurate measurement of second messenger levels underpins our ability to model subcellular compartments. We combine confocal-based sensitized emission fluorescence resonance energy transfer (FRET) to tailored data analysis to extract accurate concentrations of nuclear cAMP.

AB - Accurate measurement of second messenger levels underpins our ability to model subcellular compartments. We combine confocal-based sensitized emission fluorescence resonance energy transfer (FRET) to tailored data analysis to extract accurate concentrations of nuclear cAMP.

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M3 - Paper

AN - SCOPUS:85216028395

T2 - 2024 Laser Science, LS 2024

Y2 - 23 September 2024 through 26 September 2024

ER -

Measuring subcellular cAMP levels using FRET-based biosensing and confocal microscopy

Abstract

Conference

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