mda-5, but not RIG-I, is a common target for paramyxovirus V proteins.

K Childs; N Stock; C Ross; J Andrejeva; L Hilton; M Skinner; Richard Edward Randall; S Goodbourn

doi:10.1016/j.virol.2006.09.023

mda-5, but not RIG-I, is a common target for paramyxovirus V proteins.

K Childs, N Stock, C Ross, J Andrejeva, L Hilton, M Skinner, Richard Edward Randall, S Goodbourn

Research output: Contribution to journal › Article › peer-review

262 Citations (Scopus)

Abstract

The induction of IFN-beta by the paramyxovirus PIV5 (formerly known as SV5) is limited by the action of the viral V protein that targets the cellular RNA helicase mda-5. Here we show that 12 other paramyxoviruses also target mda-5 by a direct interaction between the conserved cysteine-rich C-terminus of their V proteins and the helicase domain of mda-5. The inhibition of IFN-beta induction is not species-restricted, being observed in a range of mammalian cells as well as in avian cells, and we show that the inhibition of mda-5 function is also not restricted to mammalian cells. In contrast, the V proteins do not bind to the related RNA helicase RIG-I and do not inhibit its activity. The relative contributions of mda-5 and RIG-I to IFN-beta induction are discussed. (c) 2006 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	190-200
Number of pages	11
Journal	Virology
Volume	359
DOIs	https://doi.org/10.1016/j.virol.2006.09.023
Publication status	Published - 1 Mar 2007

Keywords

interferon
paramyxoviruses
PIV5
mda-5
RIG-I
V proteins
DOUBLE-STRANDED-RNA
HEPATITIS-C-VIRUS
INTERFERON REGULATORY FACTOR-3
RESPIRATORY SYNCYTIAL VIRUS
NF-KAPPA-B
ANTIVIRAL SIGNALING PROTEIN
IFN-BETA PROMOTER
EPITHELIAL-CELLS
INNATE IMMUNITY
MEASLES-VIRUS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virol.2006.09.023

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title = "mda-5, but not RIG-I, is a common target for paramyxovirus V proteins.",

abstract = "The induction of IFN-beta by the paramyxovirus PIV5 (formerly known as SV5) is limited by the action of the viral V protein that targets the cellular RNA helicase mda-5. Here we show that 12 other paramyxoviruses also target mda-5 by a direct interaction between the conserved cysteine-rich C-terminus of their V proteins and the helicase domain of mda-5. The inhibition of IFN-beta induction is not species-restricted, being observed in a range of mammalian cells as well as in avian cells, and we show that the inhibition of mda-5 function is also not restricted to mammalian cells. In contrast, the V proteins do not bind to the related RNA helicase RIG-I and do not inhibit its activity. The relative contributions of mda-5 and RIG-I to IFN-beta induction are discussed. (c) 2006 Elsevier Inc. All rights reserved.",

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author = "K Childs and N Stock and C Ross and J Andrejeva and L Hilton and M Skinner and Randall, {Richard Edward} and S Goodbourn",

year = "2007",

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doi = "10.1016/j.virol.2006.09.023",

language = "English",

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journal = "Virology",

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T1 - mda-5, but not RIG-I, is a common target for paramyxovirus V proteins.

AU - Childs, K

AU - Stock, N

AU - Ross, C

AU - Andrejeva, J

AU - Hilton, L

AU - Skinner, M

AU - Randall, Richard Edward

AU - Goodbourn, S

PY - 2007/3/1

Y1 - 2007/3/1

N2 - The induction of IFN-beta by the paramyxovirus PIV5 (formerly known as SV5) is limited by the action of the viral V protein that targets the cellular RNA helicase mda-5. Here we show that 12 other paramyxoviruses also target mda-5 by a direct interaction between the conserved cysteine-rich C-terminus of their V proteins and the helicase domain of mda-5. The inhibition of IFN-beta induction is not species-restricted, being observed in a range of mammalian cells as well as in avian cells, and we show that the inhibition of mda-5 function is also not restricted to mammalian cells. In contrast, the V proteins do not bind to the related RNA helicase RIG-I and do not inhibit its activity. The relative contributions of mda-5 and RIG-I to IFN-beta induction are discussed. (c) 2006 Elsevier Inc. All rights reserved.

AB - The induction of IFN-beta by the paramyxovirus PIV5 (formerly known as SV5) is limited by the action of the viral V protein that targets the cellular RNA helicase mda-5. Here we show that 12 other paramyxoviruses also target mda-5 by a direct interaction between the conserved cysteine-rich C-terminus of their V proteins and the helicase domain of mda-5. The inhibition of IFN-beta induction is not species-restricted, being observed in a range of mammalian cells as well as in avian cells, and we show that the inhibition of mda-5 function is also not restricted to mammalian cells. In contrast, the V proteins do not bind to the related RNA helicase RIG-I and do not inhibit its activity. The relative contributions of mda-5 and RIG-I to IFN-beta induction are discussed. (c) 2006 Elsevier Inc. All rights reserved.

KW - interferon

KW - paramyxoviruses

KW - PIV5

KW - mda-5

KW - RIG-I

KW - V proteins

KW - DOUBLE-STRANDED-RNA

KW - HEPATITIS-C-VIRUS

KW - INTERFERON REGULATORY FACTOR-3

KW - RESPIRATORY SYNCYTIAL VIRUS

KW - NF-KAPPA-B

KW - ANTIVIRAL SIGNALING PROTEIN

KW - IFN-BETA PROMOTER

KW - EPITHELIAL-CELLS

KW - INNATE IMMUNITY

KW - MEASLES-VIRUS

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U2 - 10.1016/j.virol.2006.09.023

DO - 10.1016/j.virol.2006.09.023

M3 - Article

SN - 0042-6822

VL - 359

SP - 190

EP - 200

JO - Virology

JF - Virology

ER -

mda-5, but not RIG-I, is a common target for paramyxovirus V proteins.

Abstract

Keywords

UN SDGs

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