mda-5, but not RIG-I, is a common target for paramyxovirus V proteins.

K Childs, N Stock, C Ross, J Andrejeva, L Hilton, M Skinner, Richard Edward Randall, S Goodbourn

Research output: Contribution to journalArticlepeer-review

235 Citations (Scopus)

Abstract

The induction of IFN-beta by the paramyxovirus PIV5 (formerly known as SV5) is limited by the action of the viral V protein that targets the cellular RNA helicase mda-5. Here we show that 12 other paramyxoviruses also target mda-5 by a direct interaction between the conserved cysteine-rich C-terminus of their V proteins and the helicase domain of mda-5. The inhibition of IFN-beta induction is not species-restricted, being observed in a range of mammalian cells as well as in avian cells, and we show that the inhibition of mda-5 function is also not restricted to mammalian cells. In contrast, the V proteins do not bind to the related RNA helicase RIG-I and do not inhibit its activity. The relative contributions of mda-5 and RIG-I to IFN-beta induction are discussed. (c) 2006 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)190-200
Number of pages11
JournalVirology
Volume359
DOIs
Publication statusPublished - 1 Mar 2007

Keywords

  • interferon
  • paramyxoviruses
  • PIV5
  • mda-5
  • RIG-I
  • V proteins
  • DOUBLE-STRANDED-RNA
  • HEPATITIS-C-VIRUS
  • INTERFERON REGULATORY FACTOR-3
  • RESPIRATORY SYNCYTIAL VIRUS
  • NF-KAPPA-B
  • ANTIVIRAL SIGNALING PROTEIN
  • IFN-BETA PROMOTER
  • EPITHELIAL-CELLS
  • INNATE IMMUNITY
  • MEASLES-VIRUS

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