Male-specific fruitless isoforms target neurodevelopmental genes to specify a sexually dimorphic nervous system

Megan C. Neville*, Tetsuya Nojima, Elizabeth Ashley, Darren J. Parker, John Walker, Tony Southall, Bram Van de Sande, Ana C. Marques, Bettina Fischer, Andrea H. Brand, Steven Russell, Michael G. Ritchie, Stein Aerts, Stephen F. Goodwin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)

Abstract

Background

In Drosophila, male courtship behavior is regulated in large part by the gene fruitless (fru). fru encodes a set of putative transcription factors that promote male sexual behavior by controlling the development of sexually dimorphic neuronal circuitry. Little is known about how Fru proteins function at the level of transcriptional regulation or the role that isoform diversity plays in the formation of a male-specific nervous system.

Results

To characterize the roles of sex-specific Fru isoforms in specifying male behavior, we generated novel isoform-specific mutants and used a genomic approach to identify direct Fru isoform targets during development. We demonstrate that all Fru isoforms directly target genes involved in the development of the nervous system, with individual isoforms exhibiting unique binding specificities. We observe that fru behavioral phenotypes are specified by either a single isoform or a combination of isoforms. Finally, we illustrate the utility of these data for the identification of novel sexually dimorphic genomic enhancers and novel downstream regulators of male sexual behavior.

Conclusions

These findings suggest that Fru isoform diversity facilitates both redundancy and specificity in gene expression, and that the regulation of neuronal developmental genes may be the most ancient and conserved role of fru in the specification of a male-specific nervous system.

Original languageEnglish
Pages (from-to)229-241
Number of pages13
JournalCurrent Biology
Volume24
Issue number3
Early online date16 Jan 2014
DOIs
Publication statusPublished - 3 Feb 2014

Keywords

  • Antagonistic chromatin factors
  • Zinc-finger protein
  • Drosophila-melanogaster
  • Courtship song
  • Transcription factors
  • Neural circuitry
  • Behavior
  • Differentiation
  • Brain
  • Orientation

Fingerprint

Dive into the research topics of 'Male-specific fruitless isoforms target neurodevelopmental genes to specify a sexually dimorphic nervous system'. Together they form a unique fingerprint.

Cite this