Low pH-induced cytopathic effect--a survey of seven hantavirus strains

C McCaughey; Xiaohong Shi; RM Elliott; DE Wyatt; HJ O'Neill; PV Coyle

doi:10.1016/S0166-0934(99)00042-7

Low pH-induced cytopathic effect--a survey of seven hantavirus strains

C McCaughey, Xiaohong Shi, RM Elliott, DE Wyatt, HJ O'Neill, PV Coyle

School of Biology

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Hantaviruses do not produce cytopathic effects (CPE) in cell culture. However, a syncytial CPE can be induced in 7-day cultures of hantavirus growing in Vero E6 cells by reduction of the pH to approximately 6.2 using a HEPES based buffer. The appearance of this acid induced CPE was examined for seven different hantavirus strains. The differences noted were striking and reflected the taxonomic differences between hantaviruses. At 10-100 TCID50% the size of syncytial foci was very large for Seoul type viruses and smallest for Puumala viruses. The size of syncytia for Hantaan (HTN) virus was intermediate between Puumala (PUU) and Seoul (SEO) type viruses. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

Original language	English
Pages (from-to)	193-197
Number of pages	5
Journal	Journal of Virological Methods
Volume	81
Issue number	1-2
DOIs	https://doi.org/10.1016/S0166-0934(99)00042-7
Publication status	Published - Aug 1999

Keywords

HEPES (N-2-hydroxyethylpiperazine-N '-2 ethanesulfonic acid)
fusion
Vero-E6
phenotype
syncytial formation
RENAL SYNDROME HFRS
HEMORRHAGIC-FEVER
ENDOTHELIAL-CELLS
VIRUS-INFECTION
FUSION
ANTIBODY

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10.1016/S0166-0934(99)00042-7

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title = "Low pH-induced cytopathic effect--a survey of seven hantavirus strains",

abstract = "Hantaviruses do not produce cytopathic effects (CPE) in cell culture. However, a syncytial CPE can be induced in 7-day cultures of hantavirus growing in Vero E6 cells by reduction of the pH to approximately 6.2 using a HEPES based buffer. The appearance of this acid induced CPE was examined for seven different hantavirus strains. The differences noted were striking and reflected the taxonomic differences between hantaviruses. At 10-100 TCID50% the size of syncytial foci was very large for Seoul type viruses and smallest for Puumala viruses. The size of syncytia for Hantaan (HTN) virus was intermediate between Puumala (PUU) and Seoul (SEO) type viruses. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.",

keywords = "HEPES (N-2-hydroxyethylpiperazine-N '-2 ethanesulfonic acid), fusion, Vero-E6, phenotype, syncytial formation, RENAL SYNDROME HFRS, HEMORRHAGIC-FEVER, ENDOTHELIAL-CELLS, VIRUS-INFECTION, FUSION, ANTIBODY",

author = "C McCaughey and Xiaohong Shi and RM Elliott and DE Wyatt and HJ O'Neill and PV Coyle",

year = "1999",

month = aug,

doi = "10.1016/S0166-0934(99)00042-7",

language = "English",

volume = "81",

pages = "193--197",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier",

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TY - JOUR

T1 - Low pH-induced cytopathic effect--a survey of seven hantavirus strains

AU - McCaughey, C

AU - Shi, Xiaohong

AU - Elliott, RM

AU - Wyatt, DE

AU - O'Neill, HJ

AU - Coyle, PV

PY - 1999/8

Y1 - 1999/8

N2 - Hantaviruses do not produce cytopathic effects (CPE) in cell culture. However, a syncytial CPE can be induced in 7-day cultures of hantavirus growing in Vero E6 cells by reduction of the pH to approximately 6.2 using a HEPES based buffer. The appearance of this acid induced CPE was examined for seven different hantavirus strains. The differences noted were striking and reflected the taxonomic differences between hantaviruses. At 10-100 TCID50% the size of syncytial foci was very large for Seoul type viruses and smallest for Puumala viruses. The size of syncytia for Hantaan (HTN) virus was intermediate between Puumala (PUU) and Seoul (SEO) type viruses. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

AB - Hantaviruses do not produce cytopathic effects (CPE) in cell culture. However, a syncytial CPE can be induced in 7-day cultures of hantavirus growing in Vero E6 cells by reduction of the pH to approximately 6.2 using a HEPES based buffer. The appearance of this acid induced CPE was examined for seven different hantavirus strains. The differences noted were striking and reflected the taxonomic differences between hantaviruses. At 10-100 TCID50% the size of syncytial foci was very large for Seoul type viruses and smallest for Puumala viruses. The size of syncytia for Hantaan (HTN) virus was intermediate between Puumala (PUU) and Seoul (SEO) type viruses. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

KW - HEPES (N-2-hydroxyethylpiperazine-N '-2 ethanesulfonic acid)

KW - fusion

KW - Vero-E6

KW - phenotype

KW - syncytial formation

KW - RENAL SYNDROME HFRS

KW - HEMORRHAGIC-FEVER

KW - ENDOTHELIAL-CELLS

KW - VIRUS-INFECTION

KW - FUSION

KW - ANTIBODY

UR - http://www.scopus.com/inward/record.url?scp=0032799921&partnerID=8YFLogxK

UR - http://www.ncbi.nlm.nih.gov/pubmed/10488778?ordinalpos=9&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum

U2 - 10.1016/S0166-0934(99)00042-7

DO - 10.1016/S0166-0934(99)00042-7

M3 - Article

SN - 0166-0934

VL - 81

SP - 193

EP - 197

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1-2

ER -

Low pH-induced cytopathic effect--a survey of seven hantavirus strains

Abstract

Keywords

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