Long-term whole blood DNA preservation by cost-efficient cryosilicification

Liang Zhou; Qi Lei; Jimin Guo; Yuanyuan Gao; Jianjun Shi; Hong Yu; Wenxiang Yin; Jiangfan Cao; Botao Xiao; Jacopo Andreo; Romy Ettlinger; C Jeffrey Brinker; Stefan Wuttke; Wei Zhu

doi:10.1038/s41467-022-33759-y

Long-term whole blood DNA preservation by cost-efficient cryosilicification

Liang Zhou, Qi Lei, Jimin Guo, Yuanyuan Gao, Jianjun Shi, Hong Yu, Wenxiang Yin, Jiangfan Cao, Botao Xiao, Jacopo Andreo, Romy Ettlinger, C Jeffrey Brinker, Stefan Wuttke^*, Wei Zhu^*

^*Corresponding author for this work

School of Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Deoxyribonucleic acid (DNA) is the blueprint of life, and cost-effective methods for its long-term storage could have many potential benefits to society. Here we present the method of in situ cryosilicification of whole blood cells, which allows long-term preservation of DNA. Importantly, our straightforward approach is inexpensive, reliable, and yields cryosilicified samples that fulfill the essential criteria for safe, long-term DNA preservation, namely robustness against external stressors, such as radical oxygen species or ultraviolet radiation, and long-term stability in humid conditions at elevated temperatures. Our approach could enable the room temperature storage of genomic information in book-size format for more than one thousand years (thermally equivalent), costing only 0.5 $/person. Additionally, our demonstration of 3D-printed DNA banking artefacts, could potentially allow 'artificial fossilization'.

Original language	English
Article number	6265
Number of pages	14
Journal	Nature Communications
Volume	13
DOIs	https://doi.org/10.1038/s41467-022-33759-y
Publication status	Published - 21 Oct 2022

Keywords

Preservation, Biological - methods
Blood Preservation - methods
Oxygen
Ultraviolet Rays
DNA - genetics
Humans

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10.1038/s41467-022-33759-yLicence: CC BY

Zhou_2022_NC_Long-term-whole-blood_CC
Copyright © The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Final published version, 2.65 MBLicence: CC BY

Whole Blood DNA Preservation (dataset)
Ettlinger, R. (Creator), NCBI GenBank, 2022
https://www.ncbi.nlm.nih.gov/bioproject/878673
Dataset

Cite this

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title = "Long-term whole blood DNA preservation by cost-efficient cryosilicification",

abstract = "Deoxyribonucleic acid (DNA) is the blueprint of life, and cost-effective methods for its long-term storage could have many potential benefits to society. Here we present the method of in situ cryosilicification of whole blood cells, which allows long-term preservation of DNA. Importantly, our straightforward approach is inexpensive, reliable, and yields cryosilicified samples that fulfill the essential criteria for safe, long-term DNA preservation, namely robustness against external stressors, such as radical oxygen species or ultraviolet radiation, and long-term stability in humid conditions at elevated temperatures. Our approach could enable the room temperature storage of genomic information in book-size format for more than one thousand years (thermally equivalent), costing only 0.5 $/person. Additionally, our demonstration of 3D-printed DNA banking artefacts, could potentially allow 'artificial fossilization'.",

keywords = "Preservation, Biological - methods, Blood Preservation - methods, Oxygen, Ultraviolet Rays, DNA - genetics, Humans",

author = "Liang Zhou and Qi Lei and Jimin Guo and Yuanyuan Gao and Jianjun Shi and Hong Yu and Wenxiang Yin and Jiangfan Cao and Botao Xiao and Jacopo Andreo and Romy Ettlinger and {Jeffrey Brinker}, C and Stefan Wuttke and Wei Zhu",

note = "Funding: This work was supported by the National Natural Science Foundation of China (21972047 to W.Z., 52003086 to Q.L.), Guangdong Provincial Pearl River Talents Program (2019QN01Y314 to Q.L.), the Program for Guangdong Introducing Innovative and Entrepreneurial Teams (2019ZT08Y318 to W.Z.), Natural Science Foundation of Guangdong Province, China (2021A1515010724 to Q.L.), China Postdoctoral Science Foundation (2020M672625, 2021T140213 to Q.L.), Science and Technology Project of Guangzhou, China (202102020352 to W.Z., 202102020259 to Q.L.), the Fundamental Research Funds for the Central Universities of China. The authors thank the support from the Guangzhou Women and Children{\textquoteright}s Medical Center and Laboratory Animal Research Center of the South China University of Technology. S.W. acknowledges funding from the Basque Government Industry Department under the ELKARTEK and HAZITEK programs.",

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doi = "10.1038/s41467-022-33759-y",

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journal = "Nature Communications",

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T1 - Long-term whole blood DNA preservation by cost-efficient cryosilicification

AU - Zhou, Liang

AU - Lei, Qi

AU - Guo, Jimin

AU - Gao, Yuanyuan

AU - Shi, Jianjun

AU - Yu, Hong

AU - Yin, Wenxiang

AU - Cao, Jiangfan

AU - Xiao, Botao

AU - Andreo, Jacopo

AU - Ettlinger, Romy

AU - Jeffrey Brinker, C

AU - Wuttke, Stefan

AU - Zhu, Wei

N1 - Funding: This work was supported by the National Natural Science Foundation of China (21972047 to W.Z., 52003086 to Q.L.), Guangdong Provincial Pearl River Talents Program (2019QN01Y314 to Q.L.), the Program for Guangdong Introducing Innovative and Entrepreneurial Teams (2019ZT08Y318 to W.Z.), Natural Science Foundation of Guangdong Province, China (2021A1515010724 to Q.L.), China Postdoctoral Science Foundation (2020M672625, 2021T140213 to Q.L.), Science and Technology Project of Guangzhou, China (202102020352 to W.Z., 202102020259 to Q.L.), the Fundamental Research Funds for the Central Universities of China. The authors thank the support from the Guangzhou Women and Children’s Medical Center and Laboratory Animal Research Center of the South China University of Technology. S.W. acknowledges funding from the Basque Government Industry Department under the ELKARTEK and HAZITEK programs.

PY - 2022/10/21

Y1 - 2022/10/21

N2 - Deoxyribonucleic acid (DNA) is the blueprint of life, and cost-effective methods for its long-term storage could have many potential benefits to society. Here we present the method of in situ cryosilicification of whole blood cells, which allows long-term preservation of DNA. Importantly, our straightforward approach is inexpensive, reliable, and yields cryosilicified samples that fulfill the essential criteria for safe, long-term DNA preservation, namely robustness against external stressors, such as radical oxygen species or ultraviolet radiation, and long-term stability in humid conditions at elevated temperatures. Our approach could enable the room temperature storage of genomic information in book-size format for more than one thousand years (thermally equivalent), costing only 0.5 $/person. Additionally, our demonstration of 3D-printed DNA banking artefacts, could potentially allow 'artificial fossilization'.

AB - Deoxyribonucleic acid (DNA) is the blueprint of life, and cost-effective methods for its long-term storage could have many potential benefits to society. Here we present the method of in situ cryosilicification of whole blood cells, which allows long-term preservation of DNA. Importantly, our straightforward approach is inexpensive, reliable, and yields cryosilicified samples that fulfill the essential criteria for safe, long-term DNA preservation, namely robustness against external stressors, such as radical oxygen species or ultraviolet radiation, and long-term stability in humid conditions at elevated temperatures. Our approach could enable the room temperature storage of genomic information in book-size format for more than one thousand years (thermally equivalent), costing only 0.5 $/person. Additionally, our demonstration of 3D-printed DNA banking artefacts, could potentially allow 'artificial fossilization'.

KW - Preservation, Biological - methods

KW - Blood Preservation - methods

KW - Oxygen

KW - Ultraviolet Rays

KW - DNA - genetics

KW - Humans

U2 - 10.1038/s41467-022-33759-y

DO - 10.1038/s41467-022-33759-y

M3 - Article

C2 - 36270991

SN - 2041-1723

VL - 13

JO - Nature Communications

JF - Nature Communications

M1 - 6265

ER -

Long-term whole blood DNA preservation by cost-efficient cryosilicification

Abstract

Keywords

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Datasets

Whole Blood DNA Preservation (dataset)

Cite this