Localization of viral proteins in plant cells: Protein tagging

Sophie Haupt*, Angelika Ziegler, Lesley Torrance

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter


Summary: This chapter describes techniques for in vivo imaging of fluorescent fusion proteins in living cells by confocal laser scanning microscopy (CLSM). Methods are provided for (i) producing the constructs for transient expression from plasmids or virus-based vectors, (ii) introduction of constructs to plant epidermal cells; (iii) imaging of the expressed proteins by CLSM and image processing, and (iv) studying the expression in the presence of agents that affect the integrity or function of cytoskeletal elements. Notes are provided to aid comprehension and indicate problems.

Original languageEnglish
Title of host publicationPlant Virology Protocols
Subtitle of host publicationFrom Viral Sequence to Protein Function
PublisherHumana Press
Number of pages11
ISBN (Print)9781588298270
Publication statusPublished - 1 Jan 2008

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Binary vectors
  • Confocal laser scanning microscopy
  • Green fluorescent protein
  • In vivo imaging
  • Microprojectile bombardment
  • Potato mop-top virus
  • Run-off transcripts
  • Virus vectors


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