Localisation of Globodera pallida FMRFamide-related peptide encoding genes using in situ hybridisation

MJ Kimber, C Fleming, A Prior, JT Jones, DW Halton, AG Maule*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

The present study employed an in situ hybridisation technique to detect the expression of a number of FMRFamide-like peptide encoding (flp) genes, previously identified from Globodera pallida, in whole-mount preparations of the J(2) stage of this worm. gpflp-1, encoding the FMRFamide-related peptide (FaRP) KSAYMRFamide, was expressed in neurones associated with the circumpharyngeal nerve ring and specifically in a number of cell bodies in the lumbar ganglia of the perianal nerve ring. The lumbar ganglia and pre-anal ganglia along with the BDU neurones and a number of cells in the retrovesicular ganglion were observed to express gpflp-2, encoding KNKFEFIRFamide. gpflp-3 (encoding KHEYLRFamide) expression was localised to the anterior ganglion and a number of paired cells posterior to the circumpharyngeal nerve ring whilst expression of gpflp-4, encoding a number of -P(G/Q)VLRFamides. was localised to the retrovesicular ganglion. No expression of gpflp-5 was observed. Identification of the reactive cells has implicated distinct roles for the FaRPs encoded on these genes in regulation of both dorsal and ventral body wall muscles, the musculature of the vulva and in the function of a number of sensory structures in both the head and tail of G. pallida. Comparison with the expression patterns of analogous genes in Caenorhabditis elegans suggests that, whilst some of the encoded peptides are conserved between nematode species, their functions therein are distinct. Furthermore, the expression of some of these genes in a number of interneurones supports the idea that FaRPs fulfil neuromodulatory as well as neurotransmitter roles. (C) 2002 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Article numberPII S0020-7519(02)00084-X
Pages (from-to)1095-1105
Number of pages11
JournalInternational Journal for Parasitology
Volume32
Issue number9
Publication statusPublished - Aug 2002

Keywords

  • Globodera pallida
  • potato cyst nematode
  • nervous system
  • neuropeptide
  • FaRP
  • flp expression
  • Caenorhabditis elegans
  • ASCARIS-SUUM NEMATODA
  • FREE-LIVING NEMATODE
  • CENTRAL-NERVOUS-SYSTEM
  • POTATO CYST NEMATODES
  • CAENORHABDITIS-ELEGANS
  • IMMUNOCYTOCHEMICAL DEMONSTRATION
  • PARASITIC NEMATODE
  • PANAGRELLUS-REDIVIVUS
  • HAEMONCHUS-CONTORTUS
  • KSAYMRFAMIDE PF3/AF8

Fingerprint

Dive into the research topics of 'Localisation of Globodera pallida FMRFamide-related peptide encoding genes using in situ hybridisation'. Together they form a unique fingerprint.

Cite this