Lipidomic profiling of plasma free fatty acids in type-1 diabetes highlights specific changes in lipid metabolism

Amelie Isabelle Sylvie Sobczak; Samantha J. Pitt; Terry K Smith; Ramzi A. Ajjan; Alan J. Stewart

doi:10.1016/j.bbalip.2020.158823

Lipidomic profiling of plasma free fatty acids in type-1 diabetes highlights specific changes in lipid metabolism

Amelie Isabelle Sylvie Sobczak
, Samantha J. Pitt
, Terry K Smith
, Ramzi A. Ajjan
, Alan J. Stewart

Research output: Contribution to journal › Article › peer-review

Abstract

Type-1 diabetes mellitus (T1DM) is associated with metabolic changes leading to alterations in glucose and lipid handling. While T1DM- associated effects on many major plasma lipids have been characterised, such effects on plasma free fatty acids (FFA) have not been fully examined. Using gas chromatography-mass spectrometry, we measured the plasma concentrations of FFA species in individuals with T1DM (n=44) and age/sex-matched healthy controls (n=44). Relationships between FFA species and various parameters were evaluated. Plasma concentrations of myristate (14:0), palmitoleate (16:1), palmitate (16:0), linoleate (18:2), oleate (18:1c9), cis-vaccenate (18:1c11), eicosapentaenoate (20:5), arachidonate (20:4) and docosahexanoate (22:6) were reduced in the T1DM group (p<0.0001 for all, except p=0.0020 for eicosapentaenoate and p=0.0068 for arachidonate); α-linolenate (18:3) and dihomo-γ- linolenate (20:3) concentrations were unchanged. Saturated/unsaturated FFA ratio, n-3/n-6 ratio, de novo lipogenesis index (palmitate (main lipogenesis product)/linoleate (only found in diet)) and elongase index (oleate/palmitoleate) were increased in the T1DM group (p=0.0166, p=0.0089, p<0.0001 and p=0.0008 respectively). The stearoyl-CoA desaturase 1 (SCD1) index 1 (palmitoleate/palmitate) and index 2 (oleate/stearate) were reduced in T1DM (p<0.0001 for all). The delta-(5)- desaturase (D5D) index (arachidonate/dihomo-γ-linolenate) was unchanged. Age and sex had no effect on plasma FFA concentrations in T1DM, while SCD1 index 1 was positively correlated (p=0.098) and elongase index negatively correlated with age (p=0.0363). HbA1c was negatively correlated with all plasma FFAs concentrations measured except α- linolenate and dihomo-γ-linolenate. Correlations were observed between plasma FFAs and cholesterol and HDL, but not LDL or diabetes duration. Collectively, these results aid our understanding of T1DM and its effects on lipid metabolism.

Original language	English
Article number	158823
Number of pages	9
Journal	Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume	1866
Issue number	1
Early online date	1 Oct 2020
DOIs	https://doi.org/10.1016/j.bbalip.2020.158823
Publication status	Published - Jan 2021

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Fatty acid metabolism
GC-MS
HbA1c
Lipid metabolism
Lipidomics
Non-esterified fatty acid

Access to Document

10.1016/j.bbalip.2020.158823Licence: CC BY

Sobczak-2020-Lipodomic-profiling-of-plasma-BBA-158823-CCBY
Copyright © 2020 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).
Final published version, 6.15 MBLicence: CC BY

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Zinc-dependant heparin neutralisation: Charecterisation of zinc-dependant heparin neutralisation by fibrinogen and histidine-rich glycoprotein.
Stewart, A. J. (PI) & Pitt, S. J. (CoI)
27/10/15 → 26/10/18
Project: Studentship
Role of zinc in controlling histidine: Role of zinc in controlling histidine-rich glycoprotein complex formation: Implications for the development of thrombotic complications.
Stewart, A. J. (PI), Naismith, J. (CoI) & Pitt, S. J. (CoI)
8/06/15 → 7/06/18
Project: Standard

Cite this

@article{6c163be171ee48ff8e2d8fbacc478344,

title = "Lipidomic profiling of plasma free fatty acids in type-1 diabetes highlights specific changes in lipid metabolism",

abstract = "Type-1 diabetes mellitus (T1DM) is associated with metabolic changes leading to alterations in glucose and lipid handling. While T1DM- associated effects on many major plasma lipids have been characterised, such effects on plasma free fatty acids (FFA) have not been fully examined. Using gas chromatography-mass spectrometry, we measured the plasma concentrations of FFA species in individuals with T1DM (n=44) and age/sex-matched healthy controls (n=44). Relationships between FFA species and various parameters were evaluated. Plasma concentrations of myristate (14:0), palmitoleate (16:1), palmitate (16:0), linoleate (18:2), oleate (18:1c9), cis-vaccenate (18:1c11), eicosapentaenoate (20:5), arachidonate (20:4) and docosahexanoate (22:6) were reduced in the T1DM group (p<0.0001 for all, except p=0.0020 for eicosapentaenoate and p=0.0068 for arachidonate); α-linolenate (18:3) and dihomo-γ- linolenate (20:3) concentrations were unchanged. Saturated/unsaturated FFA ratio, n-3/n-6 ratio, de novo lipogenesis index (palmitate (main lipogenesis product)/linoleate (only found in diet)) and elongase index (oleate/palmitoleate) were increased in the T1DM group (p=0.0166, p=0.0089, p<0.0001 and p=0.0008 respectively). The stearoyl-CoA desaturase 1 (SCD1) index 1 (palmitoleate/palmitate) and index 2 (oleate/stearate) were reduced in T1DM (p<0.0001 for all). The delta-(5)- desaturase (D5D) index (arachidonate/dihomo-γ-linolenate) was unchanged. Age and sex had no effect on plasma FFA concentrations in T1DM, while SCD1 index 1 was positively correlated (p=0.098) and elongase index negatively correlated with age (p=0.0363). HbA1c was negatively correlated with all plasma FFAs concentrations measured except α- linolenate and dihomo-γ-linolenate. Correlations were observed between plasma FFAs and cholesterol and HDL, but not LDL or diabetes duration. Collectively, these results aid our understanding of T1DM and its effects on lipid metabolism.",

keywords = "Fatty acid metabolism, GC-MS, HbA1c, Lipid metabolism, Lipidomics, Non-esterified fatty acid",

author = "Sobczak, \{Amelie Isabelle Sylvie\} and Pitt, \{Samantha J.\} and Smith, \{Terry K\} and Ajjan, \{Ramzi A.\} and Stewart, \{Alan J.\}",

note = "This research was funded by the British Heart Foundation [grant numbers PG/15/9/31270, FS/15/42/3155].",

year = "2021",

month = jan,

doi = "10.1016/j.bbalip.2020.158823",

language = "English",

volume = "1866",

journal = "Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids",

issn = "1388-1981",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Lipidomic profiling of plasma free fatty acids in type-1 diabetes highlights specific changes in lipid metabolism

AU - Sobczak, Amelie Isabelle Sylvie

AU - Pitt, Samantha J.

AU - Smith, Terry K

AU - Ajjan, Ramzi A.

AU - Stewart, Alan J.

N1 - This research was funded by the British Heart Foundation [grant numbers PG/15/9/31270, FS/15/42/3155].

PY - 2021/1

Y1 - 2021/1

N2 - Type-1 diabetes mellitus (T1DM) is associated with metabolic changes leading to alterations in glucose and lipid handling. While T1DM- associated effects on many major plasma lipids have been characterised, such effects on plasma free fatty acids (FFA) have not been fully examined. Using gas chromatography-mass spectrometry, we measured the plasma concentrations of FFA species in individuals with T1DM (n=44) and age/sex-matched healthy controls (n=44). Relationships between FFA species and various parameters were evaluated. Plasma concentrations of myristate (14:0), palmitoleate (16:1), palmitate (16:0), linoleate (18:2), oleate (18:1c9), cis-vaccenate (18:1c11), eicosapentaenoate (20:5), arachidonate (20:4) and docosahexanoate (22:6) were reduced in the T1DM group (p<0.0001 for all, except p=0.0020 for eicosapentaenoate and p=0.0068 for arachidonate); α-linolenate (18:3) and dihomo-γ- linolenate (20:3) concentrations were unchanged. Saturated/unsaturated FFA ratio, n-3/n-6 ratio, de novo lipogenesis index (palmitate (main lipogenesis product)/linoleate (only found in diet)) and elongase index (oleate/palmitoleate) were increased in the T1DM group (p=0.0166, p=0.0089, p<0.0001 and p=0.0008 respectively). The stearoyl-CoA desaturase 1 (SCD1) index 1 (palmitoleate/palmitate) and index 2 (oleate/stearate) were reduced in T1DM (p<0.0001 for all). The delta-(5)- desaturase (D5D) index (arachidonate/dihomo-γ-linolenate) was unchanged. Age and sex had no effect on plasma FFA concentrations in T1DM, while SCD1 index 1 was positively correlated (p=0.098) and elongase index negatively correlated with age (p=0.0363). HbA1c was negatively correlated with all plasma FFAs concentrations measured except α- linolenate and dihomo-γ-linolenate. Correlations were observed between plasma FFAs and cholesterol and HDL, but not LDL or diabetes duration. Collectively, these results aid our understanding of T1DM and its effects on lipid metabolism.

AB - Type-1 diabetes mellitus (T1DM) is associated with metabolic changes leading to alterations in glucose and lipid handling. While T1DM- associated effects on many major plasma lipids have been characterised, such effects on plasma free fatty acids (FFA) have not been fully examined. Using gas chromatography-mass spectrometry, we measured the plasma concentrations of FFA species in individuals with T1DM (n=44) and age/sex-matched healthy controls (n=44). Relationships between FFA species and various parameters were evaluated. Plasma concentrations of myristate (14:0), palmitoleate (16:1), palmitate (16:0), linoleate (18:2), oleate (18:1c9), cis-vaccenate (18:1c11), eicosapentaenoate (20:5), arachidonate (20:4) and docosahexanoate (22:6) were reduced in the T1DM group (p<0.0001 for all, except p=0.0020 for eicosapentaenoate and p=0.0068 for arachidonate); α-linolenate (18:3) and dihomo-γ- linolenate (20:3) concentrations were unchanged. Saturated/unsaturated FFA ratio, n-3/n-6 ratio, de novo lipogenesis index (palmitate (main lipogenesis product)/linoleate (only found in diet)) and elongase index (oleate/palmitoleate) were increased in the T1DM group (p=0.0166, p=0.0089, p<0.0001 and p=0.0008 respectively). The stearoyl-CoA desaturase 1 (SCD1) index 1 (palmitoleate/palmitate) and index 2 (oleate/stearate) were reduced in T1DM (p<0.0001 for all). The delta-(5)- desaturase (D5D) index (arachidonate/dihomo-γ-linolenate) was unchanged. Age and sex had no effect on plasma FFA concentrations in T1DM, while SCD1 index 1 was positively correlated (p=0.098) and elongase index negatively correlated with age (p=0.0363). HbA1c was negatively correlated with all plasma FFAs concentrations measured except α- linolenate and dihomo-γ-linolenate. Correlations were observed between plasma FFAs and cholesterol and HDL, but not LDL or diabetes duration. Collectively, these results aid our understanding of T1DM and its effects on lipid metabolism.

KW - Fatty acid metabolism

KW - GC-MS

KW - HbA1c

KW - Lipid metabolism

KW - Lipidomics

KW - Non-esterified fatty acid

U2 - 10.1016/j.bbalip.2020.158823

DO - 10.1016/j.bbalip.2020.158823

M3 - Article

SN - 1388-1981

VL - 1866

JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

IS - 1

M1 - 158823

ER -

Lipidomic profiling of plasma free fatty acids in type-1 diabetes highlights specific changes in lipid metabolism

Abstract

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Keywords

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Projects

Zinc-dependant heparin neutralisation: Charecterisation of zinc-dependant heparin neutralisation by fibrinogen and histidine-rich glycoprotein.

Role of zinc in controlling histidine: Role of zinc in controlling histidine-rich glycoprotein complex formation: Implications for the development of thrombotic complications.

Cite this