Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope

Marc Bathe-Peters; Philipp Gmach; Paolo Annibale; Martin J Lohse

doi:10.1016/j.dib.2019.105063

Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope

Marc Bathe-Peters, Philipp Gmach, Paolo Annibale^*, Martin J Lohse

^*Corresponding author for this work

School of Physics and Astronomy

Research output: Contribution to journal › Article › peer-review

Abstract

We report here on the measurement of the diffusion coefficient of fluorescent species using a commercial microscope possessing a resonant scanner. Sequential linescans with a rate of up to 12 kHz yield a temporal resolution of 83 μs, making the setup amenable to measure diffusion rates over a range covering at least three orders of magnitude, from 100 μm²/s down to 0.1 μm²/s. We share representative data sets covering (i) the diffusion of a dye molecule, observed in media of different viscosities and (ii) the diffusion of a prototypical membrane receptor.

The data can be valuable for researchers interested in the rapid diffusion properties of nuclear, cytosolic or membrane bound proteins fused to fluorescent tags.

Original language	English
Article number	105063
Number of pages	5
Journal	Data in Brief
Volume	29
Early online date	2 Jan 2020
DOIs	https://doi.org/10.1016/j.dib.2019.105063
Publication status	Published - Apr 2020

Keywords

Confocal microscopy
Fluorescence correlatioon spectroscopy
Member receptor
Diffusion

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10.1016/j.dib.2019.105063Licence: CC BY

Bathe-Peters_2020_DiB_Linescan-microscopy_CC
Copyright © 2020 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons. org/licenses/by/4.0/).
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Cite this

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title = "Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope",

abstract = "We report here on the measurement of the diffusion coefficient of fluorescent species using a commercial microscope possessing a resonant scanner. Sequential linescans with a rate of up to 12 kHz yield a temporal resolution of 83 μs, making the setup amenable to measure diffusion rates over a range covering at least three orders of magnitude, from 100 μm2/s down to 0.1 μm2/s. We share representative data sets covering (i) the diffusion of a dye molecule, observed in media of different viscosities and (ii) the diffusion of a prototypical membrane receptor. The data can be valuable for researchers interested in the rapid diffusion properties of nuclear, cytosolic or membrane bound proteins fused to fluorescent tags.",

keywords = "Confocal microscopy, Fluorescence correlatioon spectroscopy, Member receptor, Diffusion",

author = "Marc Bathe-Peters and Philipp Gmach and Paolo Annibale and Lohse, {Martin J}",

note = "We are grateful to the Max Delbr{\"u}ck Center for Molecular Medicine in the Helmholtz Association for core support and funding. P.A. and M.J.L. would like to acknowledge funding from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Project-ID 421152132-SFB1423 subproject C03. ",

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doi = "10.1016/j.dib.2019.105063",

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AU - Bathe-Peters, Marc

AU - Gmach, Philipp

AU - Annibale, Paolo

AU - Lohse, Martin J

N1 - We are grateful to the Max Delbrück Center for Molecular Medicine in the Helmholtz Association for core support and funding. P.A. and M.J.L. would like to acknowledge funding from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Project-ID 421152132-SFB1423 subproject C03.

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N2 - We report here on the measurement of the diffusion coefficient of fluorescent species using a commercial microscope possessing a resonant scanner. Sequential linescans with a rate of up to 12 kHz yield a temporal resolution of 83 μs, making the setup amenable to measure diffusion rates over a range covering at least three orders of magnitude, from 100 μm2/s down to 0.1 μm2/s. We share representative data sets covering (i) the diffusion of a dye molecule, observed in media of different viscosities and (ii) the diffusion of a prototypical membrane receptor. The data can be valuable for researchers interested in the rapid diffusion properties of nuclear, cytosolic or membrane bound proteins fused to fluorescent tags.

AB - We report here on the measurement of the diffusion coefficient of fluorescent species using a commercial microscope possessing a resonant scanner. Sequential linescans with a rate of up to 12 kHz yield a temporal resolution of 83 μs, making the setup amenable to measure diffusion rates over a range covering at least three orders of magnitude, from 100 μm2/s down to 0.1 μm2/s. We share representative data sets covering (i) the diffusion of a dye molecule, observed in media of different viscosities and (ii) the diffusion of a prototypical membrane receptor. The data can be valuable for researchers interested in the rapid diffusion properties of nuclear, cytosolic or membrane bound proteins fused to fluorescent tags.

KW - Confocal microscopy

KW - Fluorescence correlatioon spectroscopy

KW - Member receptor

KW - Diffusion

U2 - 10.1016/j.dib.2019.105063

DO - 10.1016/j.dib.2019.105063

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VL - 29

JO - Data in Brief

JF - Data in Brief

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Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope

Abstract

Keywords

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