Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope

Marc Bathe-Peters, Philipp Gmach, Paolo Annibale*, Martin J Lohse

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

We report here on the measurement of the diffusion coefficient of fluorescent species using a commercial microscope possessing a resonant scanner. Sequential linescans with a rate of up to 12 kHz yield a temporal resolution of 83 μs, making the setup amenable to measure diffusion rates over a range covering at least three orders of magnitude, from 100 μm2/s down to 0.1 μm2/s. We share representative data sets covering (i) the diffusion of a dye molecule, observed in media of different viscosities and (ii) the diffusion of a prototypical membrane receptor. 

The data can be valuable for researchers interested in the rapid diffusion properties of nuclear, cytosolic or membrane bound proteins fused to fluorescent tags.

Original languageEnglish
Article number105063
Number of pages5
JournalData in Brief
Volume29
Early online date2 Jan 2020
DOIs
Publication statusPublished - Apr 2020

Keywords

  • Confocal microscopy
  • Fluorescence correlatioon spectroscopy
  • Member receptor
  • Diffusion

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