Linear Eyring plots conceal a change in rate-limiting step in an enzyme reaction

Teresa Filipa Guerreiro Machado, Tracey Gloster, Rafael Guimaraes da Silva

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


The temperature dependence of psychrophilic and mesophilic (R)-3-hydroxybutyrate dehydrogenase steady-state rates yields nonlinear and linear Eyring plots, respectively. Solvent viscosity effects and multiple- and single-turnover pre-steady-state kinetics demonstrate that while product release is rate-limiting at high temperatures for the psychrophilic enzyme, either interconversion between enzyme-substrates and enzyme-products complexes or a step prior to it limits the rate at low temperature. Unexpectedly, a similar change in rate-limiting step is observed with the mesophilic en-zyme, where a step prior to chemistry becomes rate-limiting at low temperature. This observation may have implica-tions for past and future interpretation of temperature-rate profiles.
Original languageEnglish
Pages (from-to)6757-6761
Issue number49
Early online date26 Nov 2018
Publication statusPublished - 11 Dec 2018


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