Light sheet microscopy with acoustic sample confinement

Zhengyi Yang; Katy L.H. Cole; Yongqiang Qiu; Ildiko Maureen Lara Somorjai; Philip Wijesinghe; Jonathan Nylk; Sandy Cochran; Gabriel C. Spalding; David A. Lyons; Kishan Dholakia

doi:10.1038/s41467-019-08514-5

Light sheet microscopy with acoustic sample confinement

Zhengyi Yang, Katy L.H. Cole, Yongqiang Qiu, Ildiko Maureen Lara Somorjai, Philip Wijesinghe, Jonathan Nylk, Sandy Cochran, Gabriel C. Spalding, David A. Lyons, Kishan Dholakia

Research output: Contribution to journal › Article › peer-review

Abstract

Contactless sample confinement would enable a whole host of new studies in developmental biology and neuroscience, in particular, when combined with long-term, wide-field optical imaging. To achieve this goal, we demonstrate a contactless acoustic gradient force trap for sample confinement in light sheet microscopy. Our approach allows the integration of real-time environmentally controlled experiments with wide-field low photo-toxic imaging, which we demonstrate on a variety of marine animal embryos and larvae. To illustrate the key advantages of our approach, we provide quantitative data for the dynamic response of the heartbeat of zebrafish larvae to verapamil and norepinephrine, which are known to affect cardiovascular function. Optical flow analysis allows us to explore the cardiac cycle of the zebrafish and determine the changes in contractile volume within the heart. Overcoming the restrictions of sample immobilisation and mounting can open up a broad range of studies, with real-time drug-based assays and biomechanical analyses.

Original language	English
Article number	669
Number of pages	8
Journal	Nature Communications
Volume	10
DOIs	https://doi.org/10.1038/s41467-019-08514-5
Publication status	Published - 8 Feb 2019

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Final published version, 2.93 MBLicence: CC BY

M Squared - USTAN Biophotonics Nexus: M Sqaured - St Andrews Biophotonics Nexus
Dholakia, K. (PI)
EPSRC
1/11/17 → 31/10/22
Project: Standard
Resonant and shaped photonics for under: Resonant and shaped photonics for understanding the physical and biomedical world
Dholakia, K. (PI) & Gather, M. C. (CoI)
1/08/17 → 31/07/22
Project: Standard
Dr Ildiko: ISSF - Wellcome Trust
Somorjai, I. M. L. (PI)
The Wellcome Trust
15/05/17 → 14/02/18
Project: Standard

Kishan Dholakia
- kd1st-andrews.acuk
Person: Academic
Ildiko Maureen Lara Somorjai
- imlsst-andrews.acuk
Person: Academic

Data underpinning: Light sheet microscopy with acoustic sample confinement
Nylk, J. (Creator), Yang, Z. (Creator), Cole, K. (Creator), Qiu, Y. (Creator), Somorjai, I. M. L. (Creator), Wijesinghe, P. (Creator), Cochran, S. (Creator), Spalding, G. (Creator), Lyons, D. (Creator) & Dholakia, K. (Creator), University of St Andrews, 27 Apr 2022
DOI: 10.17630/cc36c86a-5ed5-4198-8c93-52310dcf979c
Dataset

File

Cite this

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title = "Light sheet microscopy with acoustic sample confinement",

abstract = "Contactless sample confinement would enable a whole host of new studies in developmental biology and neuroscience, in particular, when combined with long-term, wide-field optical imaging. To achieve this goal, we demonstrate a contactless acoustic gradient force trap for sample confinement in light sheet microscopy. Our approach allows the integration of real-time environmentally controlled experiments with wide-field low photo-toxic imaging, which we demonstrate on a variety of marine animal embryos and larvae. To illustrate the key advantages of our approach, we provide quantitative data for the dynamic response of the heartbeat of zebrafish larvae to verapamil and norepinephrine, which are known to affect cardiovascular function. Optical flow analysis allows us to explore the cardiac cycle of the zebrafish and determine the changes in contractile volume within the heart. Overcoming the restrictions of sample immobilisation and mounting can open up a broad range of studies, with real-time drug-based assays and biomechanical analyses.",

author = "Zhengyi Yang and Cole, {Katy L.H.} and Yongqiang Qiu and Somorjai, {Ildiko Maureen Lara} and Philip Wijesinghe and Jonathan Nylk and Sandy Cochran and Spalding, {Gabriel C.} and Lyons, {David A.} and Kishan Dholakia",

note = "Funding: UK Engineering and Physical Sciences Research Council (EPSRC) (grant numbers EP/P030017/1, EP/M000869/1 and EP/R004854/1) for funding. Work in the Somorjai lab is supported by the Wellcome Trust ISSF, the RS Macdonald Trust Charitable and EU Horizon 2020 INFRADEV ”CORBEL” (grant number 654248).",

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AU - Yang, Zhengyi

AU - Cole, Katy L.H.

AU - Qiu, Yongqiang

AU - Somorjai, Ildiko Maureen Lara

AU - Wijesinghe, Philip

AU - Nylk, Jonathan

AU - Cochran, Sandy

AU - Spalding, Gabriel C.

AU - Lyons, David A.

AU - Dholakia, Kishan

N1 - Funding: UK Engineering and Physical Sciences Research Council (EPSRC) (grant numbers EP/P030017/1, EP/M000869/1 and EP/R004854/1) for funding. Work in the Somorjai lab is supported by the Wellcome Trust ISSF, the RS Macdonald Trust Charitable and EU Horizon 2020 INFRADEV ”CORBEL” (grant number 654248).

PY - 2019/2/8

Y1 - 2019/2/8

N2 - Contactless sample confinement would enable a whole host of new studies in developmental biology and neuroscience, in particular, when combined with long-term, wide-field optical imaging. To achieve this goal, we demonstrate a contactless acoustic gradient force trap for sample confinement in light sheet microscopy. Our approach allows the integration of real-time environmentally controlled experiments with wide-field low photo-toxic imaging, which we demonstrate on a variety of marine animal embryos and larvae. To illustrate the key advantages of our approach, we provide quantitative data for the dynamic response of the heartbeat of zebrafish larvae to verapamil and norepinephrine, which are known to affect cardiovascular function. Optical flow analysis allows us to explore the cardiac cycle of the zebrafish and determine the changes in contractile volume within the heart. Overcoming the restrictions of sample immobilisation and mounting can open up a broad range of studies, with real-time drug-based assays and biomechanical analyses.

AB - Contactless sample confinement would enable a whole host of new studies in developmental biology and neuroscience, in particular, when combined with long-term, wide-field optical imaging. To achieve this goal, we demonstrate a contactless acoustic gradient force trap for sample confinement in light sheet microscopy. Our approach allows the integration of real-time environmentally controlled experiments with wide-field low photo-toxic imaging, which we demonstrate on a variety of marine animal embryos and larvae. To illustrate the key advantages of our approach, we provide quantitative data for the dynamic response of the heartbeat of zebrafish larvae to verapamil and norepinephrine, which are known to affect cardiovascular function. Optical flow analysis allows us to explore the cardiac cycle of the zebrafish and determine the changes in contractile volume within the heart. Overcoming the restrictions of sample immobilisation and mounting can open up a broad range of studies, with real-time drug-based assays and biomechanical analyses.

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M3 - Article

SN - 2041-1723

VL - 10

JO - Nature Communications

JF - Nature Communications

M1 - 669

ER -

Light sheet microscopy with acoustic sample confinement

Abstract

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Projects

M Squared - USTAN Biophotonics Nexus: M Sqaured - St Andrews Biophotonics Nexus

Resonant and shaped photonics for under: Resonant and shaped photonics for understanding the physical and biomedical world

Dr Ildiko: ISSF - Wellcome Trust

Profiles

Kishan Dholakia

Ildiko Maureen Lara Somorjai

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Data underpinning: Light sheet microscopy with acoustic sample confinement

Cite this