Light-sheet microscopy using an Airy beam

Tom Vettenburg; Heather Isla Campbell Dalgarno; Jonathan Nylk; Clara Coll-Lladó; David Ellard Keith Ferrier; Tomáš  Čižmár; Frank J Gunn-Moore; Kishan Dholakia

doi:10.1038/nmeth.2922

Light-sheet microscopy using an Airy beam

Tom Vettenburg, Heather Isla Campbell Dalgarno, Jonathan Nylk, Clara Coll-Lladó, David Ellard Keith Ferrier, Tomáš Čižmár, Frank J Gunn-Moore, Kishan Dholakia

Research output: Contribution to journal › Article › peer-review

Abstract

Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.

Original language	English
Pages (from-to)	541-544
Number of pages	7
Journal	Nature Methods
Volume	11
Issue number	5
Early online date	6 Apr 2014
DOIs	https://doi.org/10.1038/nmeth.2922
Publication status	Published - May 2014

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10.1038/nmeth.2922

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title = "Light-sheet microscopy using an Airy beam",

abstract = "Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.",

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note = "The project was supported by the UK Engineering and Physical Sciences Research Council, RS Macdonald Charitable Trust, Scottish Universities Life Sciences Alliance and St Andrews 600 anniversary BRAINS appeal. The PhD apprenticeship of C.C.L. was funded by the School of Biology. ",

year = "2014",

month = may,

doi = "10.1038/nmeth.2922",

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volume = "11",

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journal = "Nature Methods",

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T1 - Light-sheet microscopy using an Airy beam

AU - Vettenburg, Tom

AU - Dalgarno, Heather Isla Campbell

AU - Nylk, Jonathan

AU - Coll-Lladó, Clara

AU - Ferrier, David Ellard Keith

AU - Čižmár, Tomáš

AU - Gunn-Moore, Frank J

AU - Dholakia, Kishan

N1 - The project was supported by the UK Engineering and Physical Sciences Research Council, RS Macdonald Charitable Trust, Scottish Universities Life Sciences Alliance and St Andrews 600 anniversary BRAINS appeal. The PhD apprenticeship of C.C.L. was funded by the School of Biology.

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N2 - Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.

AB - Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.

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JO - Nature Methods

JF - Nature Methods

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ER -

Light-sheet microscopy using an Airy beam

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Kishan Dholakia

Frank J Gunn-Moore

Evolution of muscle regulatory genes in chordates

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Light-sheet microscopy using an Airy beam

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Kishan Dholakia

Frank J Gunn-Moore

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Evolution of muscle regulatory genes in chordates

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