Abstract
Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.
Original language | English |
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Pages (from-to) | 541-544 |
Number of pages | 7 |
Journal | Nature Methods |
Volume | 11 |
Issue number | 5 |
Early online date | 6 Apr 2014 |
DOIs | |
Publication status | Published - May 2014 |
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Frank J Gunn-Moore
- School of Biology - Co Director of the MacKenzie Institute (Science), Professor
- Sir James Mackenzie Institute for Early Diagnosis
- Centre for Biophotonics
- Institute of Behavioural and Neural Sciences
- Biomedical Sciences Research Complex
Person: Academic