Lasing within live cells containing intracellular optical microresonators for barcode-type cell tagging and tracking

Marcel Schubert; Anja Steude; Philipp Liehm; Nils Michael Kronenberg; Markus Karl; Elaine Catherine Campbell; Simon John Powis; Malte Christian Gather

doi:10.1021/acs.nanolett.5b02491

Lasing within live cells containing intracellular optical microresonators for barcode-type cell tagging and tracking

Marcel Schubert, Anja Steude, Philipp Liehm, Nils Michael Kronenberg, Markus Karl, Elaine Catherine Campbell, Simon John Powis, Malte Christian Gather

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Abstract

We report on a laser that is fully embedded into a single live cell. By harnessing natural endocytosis of the cell we introduce a fluorescent whispering gallery mode (WGM) micro-resonator into the cell cytoplasm. On pumping with nanojoule light pulses, green laser emission is generated inside the cells. Our approach can be applied to different cell types and cells with micro-resonators remain viable for weeks under standard conditions. The characteristics of the lasing spectrum provide each cell with a barcode-type label which enables uniquely identifying and tracking of individual migrating cells. Self-sustained lasing from cells paves the way to new forms of cell tracking, intracellular sensing and adaptive imaging.

Original language	English
Pages (from-to)	5647-5652
Number of pages	6
Journal	Nano Letters
Volume	15
Issue number	8
Early online date	17 Jul 2015
DOIs	https://doi.org/10.1021/acs.nanolett.5b02491
Publication status	Published - 12 Aug 2015

Keywords

Biological lasers
Intracellular lasing
Whispering Gallery Mode resonator
Opsonized beads
Multiplexing
Cell tracking and tagging

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10.1021/acs.nanolett.5b02491

schubert m, intracellular lasers revised2
Copyright 2015 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Nano Letters, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/acs.nanolett.5b02491
Accepted author manuscript, 1.06 MB

EU FP7 Marie Curie CIG BioLAS: EU FP7 Marie Curie CIG BioLAS
Gather, M. C. (PI)
European Commission
1/08/13 → 31/07/17
Project: Standard

Cite this

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title = "Lasing within live cells containing intracellular optical microresonators for barcode-type cell tagging and tracking",

abstract = "We report on a laser that is fully embedded into a single live cell. By harnessing natural endocytosis of the cell we introduce a fluorescent whispering gallery mode (WGM) micro-resonator into the cell cytoplasm. On pumping with nanojoule light pulses, green laser emission is generated inside the cells. Our approach can be applied to different cell types and cells with micro-resonators remain viable for weeks under standard conditions. The characteristics of the lasing spectrum provide each cell with a barcode-type label which enables uniquely identifying and tracking of individual migrating cells. Self-sustained lasing from cells paves the way to new forms of cell tracking, intracellular sensing and adaptive imaging. ",

keywords = "Biological lasers, Intracellular lasing, Whispering Gallery Mode resonator, Opsonized beads, Multiplexing, Cell tracking and tagging",

author = "Marcel Schubert and Anja Steude and Philipp Liehm and Kronenberg, {Nils Michael} and Markus Karl and Campbell, {Elaine Catherine} and Powis, {Simon John} and Gather, {Malte Christian}",

note = "This work was supported by the European Union Marie Curie Career Integration Grant (PCIG12-GA-2012-334407) and the Scottish Funding Council (SUPA II). M.S. acknowledges funding by the German Science Foundation (DFG) through a Research Fellowship (SCHU 3003/1-1).",

year = "2015",

month = aug,

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doi = "10.1021/acs.nanolett.5b02491",

language = "English",

volume = "15",

pages = "5647--5652",

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T1 - Lasing within live cells containing intracellular optical microresonators for barcode-type cell tagging and tracking

AU - Schubert, Marcel

AU - Steude, Anja

AU - Liehm, Philipp

AU - Kronenberg, Nils Michael

AU - Karl, Markus

AU - Campbell, Elaine Catherine

AU - Powis, Simon John

AU - Gather, Malte Christian

N1 - This work was supported by the European Union Marie Curie Career Integration Grant (PCIG12-GA-2012-334407) and the Scottish Funding Council (SUPA II). M.S. acknowledges funding by the German Science Foundation (DFG) through a Research Fellowship (SCHU 3003/1-1).

PY - 2015/8/12

Y1 - 2015/8/12

N2 - We report on a laser that is fully embedded into a single live cell. By harnessing natural endocytosis of the cell we introduce a fluorescent whispering gallery mode (WGM) micro-resonator into the cell cytoplasm. On pumping with nanojoule light pulses, green laser emission is generated inside the cells. Our approach can be applied to different cell types and cells with micro-resonators remain viable for weeks under standard conditions. The characteristics of the lasing spectrum provide each cell with a barcode-type label which enables uniquely identifying and tracking of individual migrating cells. Self-sustained lasing from cells paves the way to new forms of cell tracking, intracellular sensing and adaptive imaging.

AB - We report on a laser that is fully embedded into a single live cell. By harnessing natural endocytosis of the cell we introduce a fluorescent whispering gallery mode (WGM) micro-resonator into the cell cytoplasm. On pumping with nanojoule light pulses, green laser emission is generated inside the cells. Our approach can be applied to different cell types and cells with micro-resonators remain viable for weeks under standard conditions. The characteristics of the lasing spectrum provide each cell with a barcode-type label which enables uniquely identifying and tracking of individual migrating cells. Self-sustained lasing from cells paves the way to new forms of cell tracking, intracellular sensing and adaptive imaging.

KW - Biological lasers

KW - Intracellular lasing

KW - Whispering Gallery Mode resonator

KW - Opsonized beads

KW - Multiplexing

KW - Cell tracking and tagging

U2 - 10.1021/acs.nanolett.5b02491

DO - 10.1021/acs.nanolett.5b02491

M3 - Article

SN - 1530-6984

VL - 15

SP - 5647

EP - 5652

JO - Nano Letters

JF - Nano Letters

IS - 8

ER -

Lasing within live cells containing intracellular optical microresonators for barcode-type cell tagging and tracking

Abstract

Keywords

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Projects

EU FP7 Marie Curie CIG BioLAS: EU FP7 Marie Curie CIG BioLAS

Profiles

Malte Christian Gather

Simon John Powis

Cite this