Lasing within live cells containing intracellular optical microresonators for barcode-type cell tagging and tracking

Marcel Schubert, Anja Steude, Philipp Liehm, Nils Michael Kronenberg, Markus Karl, Elaine Catherine Campbell, Simon John Powis, Malte Christian Gather

Research output: Contribution to journalArticlepeer-review

114 Citations (Scopus)
5 Downloads (Pure)

Abstract

We report on a laser that is fully embedded into a single live cell. By harnessing natural endocytosis of the cell we introduce a fluorescent whispering gallery mode (WGM) micro-resonator into the cell cytoplasm. On pumping with nanojoule light pulses, green laser emission is generated inside the cells. Our approach can be applied to different cell types and cells with micro-resonators remain viable for weeks under standard conditions. The characteristics of the lasing spectrum provide each cell with a barcode-type label which enables uniquely identifying and tracking of individual migrating cells. Self-sustained lasing from cells paves the way to new forms of cell tracking, intracellular sensing and adaptive imaging.
Original languageEnglish
Pages (from-to)5647-5652
Number of pages6
JournalNano Letters
Volume15
Issue number8
Early online date17 Jul 2015
DOIs
Publication statusPublished - 12 Aug 2015

Keywords

  • Biological lasers
  • Intracellular lasing
  • Whispering Gallery Mode resonator
  • Opsonized beads
  • Multiplexing
  • Cell tracking and tagging

Fingerprint

Dive into the research topics of 'Lasing within live cells containing intracellular optical microresonators for barcode-type cell tagging and tracking'. Together they form a unique fingerprint.

Cite this