Lasing in live mitotic and non-phagocytic cells by efficient delivery of microresonators

Marcel Schubert; Klara Volckaert; Markus Karl; Andrew Morton; Philipp Liehm; Gareth Brian Miles; Simon John Powis; Malte Christian Gather

doi:10.1038/srep40877

Lasing in live mitotic and non-phagocytic cells by efficient delivery of microresonators

Marcel Schubert
, Klara Volckaert
, Markus Karl
, Andrew Morton
, Philipp Liehm
, Gareth Brian Miles
, Simon John Powis
, Malte Christian Gather

Research output: Contribution to journal › Article › peer-review

Abstract

Reliable methods to individually track large numbers of cells in real time are urgently needed to advance our understanding of important biological processes like cancer metastasis, neuronal network development and wound healing. It has recently been suggested to introduce microscopic whispering gallery mode lasers into the cytoplasm of cells and to use their characteristic, size dependent emission spectrum as optical barcode but so far there is no evidence that this approach is generally applicable. Here, we describe a method that drastically improves intracellular delivery of resonators for several cell types, including mitotic and non-phagocytic cells. In addition, we characterize the influence of resonator size on the spectral characteristics of the emitted laser light and identify an optimum size range that facilitates tagging and tracking of thousands of cells simultaneously. Finally, we observe that the microresonators remain internalized by cells during cell division, which enables tagging several generations of cells.

Original language	English
Article number	40877
Journal	Scientific Reports
Volume	7
DOIs	https://doi.org/10.1038/srep40877
Publication status	Published - 19 Jan 2017

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10.1038/srep40877Licence: CC BY

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Copyright the Authors 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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Living Lasers: H2020 Marie Curie Fellowship 2014 LIVING LASERS
Gather, M. (PI)
Joint Research Centre European Commission
1/08/15 → 31/07/17
Project: Fellowship
H2020 ERC Starting Grant ABLASE: H2020 ERC Starting Grant ABLASE (Grant Agreement No. 640012)
Gather, M. (PI)
European Research Council
1/06/15 → 31/05/21
Project: Standard
EU FP7 Marie Curie CIG BioLAS: EU FP7 Marie Curie CIG BioLAS
Gather, M. (PI)
Joint Research Centre European Commission
1/08/13 → 31/07/17
Project: Standard

Data underpinning - Lasing in Live Mitotic and Non-Phagocytic Cells by Efficient Delivery of Microresonators
Schubert, M. (Creator), Volckaert, K. (Creator), Karl, M. (Creator), Morton, A. (Creator), Liehm, P. (Creator), Miles, G. B. (Creator), Powis, S. J. (Creator) & Gather, M. C. (Creator), University of St Andrews, 19 Jan 2017
DOI: 10.17630/ec246742-c392-41f8-865d-31ad86801a63
Dataset

File

Living lasers: lasing from biological and biocompatible soft matter
Karl, M. (Author), Gather, M. (Supervisor) & Turnbull, G. (Supervisor), 6 Dec 2018
Student thesis: Doctoral Thesis (PhD)

Cite this

@article{8a73d3d6456c47d6b39b8c7a2965d0c9,

title = "Lasing in live mitotic and non-phagocytic cells by efficient delivery of microresonators",

abstract = "Reliable methods to individually track large numbers of cells in real time are urgently needed to advance our understanding of important biological processes like cancer metastasis, neuronal network development and wound healing. It has recently been suggested to introduce microscopic whispering gallery mode lasers into the cytoplasm of cells and to use their characteristic, size dependent emission spectrum as optical barcode but so far there is no evidence that this approach is generally applicable. Here, we describe a method that drastically improves intracellular delivery of resonators for several cell types, including mitotic and non-phagocytic cells. In addition, we characterize the influence of resonator size on the spectral characteristics of the emitted laser light and identify an optimum size range that facilitates tagging and tracking of thousands of cells simultaneously. Finally, we observe that the microresonators remain internalized by cells during cell division, which enables tagging several generations of cells.",

author = "Marcel Schubert and Klara Volckaert and Markus Karl and Andrew Morton and Philipp Liehm and Miles, \{Gareth Brian\} and Powis, \{Simon John\} and Gather, \{Malte Christian\}",

note = "We acknowledge support from the ERC Starting Grant ABLASE (640012), the Scottish Funding Council (via SUPA), and the European Union Marie Curie Career Integration Grant (PCIG12-GA-2012-334407). MS acknowledges funding by the European Commission through a Marie Sklodowska-Curie Individual Fellowship (659213). MK and PL acknowledge funding from the EPSRC DTG (EP/M506631/1 and EP/L505079/1).",

year = "2017",

month = jan,

day = "19",

doi = "10.1038/srep40877",

language = "English",

volume = "7",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Research",

}

TY - JOUR

T1 - Lasing in live mitotic and non-phagocytic cells by efficient delivery of microresonators

AU - Schubert, Marcel

AU - Volckaert, Klara

AU - Karl, Markus

AU - Morton, Andrew

AU - Liehm, Philipp

AU - Miles, Gareth Brian

AU - Powis, Simon John

AU - Gather, Malte Christian

N1 - We acknowledge support from the ERC Starting Grant ABLASE (640012), the Scottish Funding Council (via SUPA), and the European Union Marie Curie Career Integration Grant (PCIG12-GA-2012-334407). MS acknowledges funding by the European Commission through a Marie Sklodowska-Curie Individual Fellowship (659213). MK and PL acknowledge funding from the EPSRC DTG (EP/M506631/1 and EP/L505079/1).

PY - 2017/1/19

Y1 - 2017/1/19

N2 - Reliable methods to individually track large numbers of cells in real time are urgently needed to advance our understanding of important biological processes like cancer metastasis, neuronal network development and wound healing. It has recently been suggested to introduce microscopic whispering gallery mode lasers into the cytoplasm of cells and to use their characteristic, size dependent emission spectrum as optical barcode but so far there is no evidence that this approach is generally applicable. Here, we describe a method that drastically improves intracellular delivery of resonators for several cell types, including mitotic and non-phagocytic cells. In addition, we characterize the influence of resonator size on the spectral characteristics of the emitted laser light and identify an optimum size range that facilitates tagging and tracking of thousands of cells simultaneously. Finally, we observe that the microresonators remain internalized by cells during cell division, which enables tagging several generations of cells.

AB - Reliable methods to individually track large numbers of cells in real time are urgently needed to advance our understanding of important biological processes like cancer metastasis, neuronal network development and wound healing. It has recently been suggested to introduce microscopic whispering gallery mode lasers into the cytoplasm of cells and to use their characteristic, size dependent emission spectrum as optical barcode but so far there is no evidence that this approach is generally applicable. Here, we describe a method that drastically improves intracellular delivery of resonators for several cell types, including mitotic and non-phagocytic cells. In addition, we characterize the influence of resonator size on the spectral characteristics of the emitted laser light and identify an optimum size range that facilitates tagging and tracking of thousands of cells simultaneously. Finally, we observe that the microresonators remain internalized by cells during cell division, which enables tagging several generations of cells.

U2 - 10.1038/srep40877

DO - 10.1038/srep40877

M3 - Article

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

M1 - 40877

ER -

Lasing in live mitotic and non-phagocytic cells by efficient delivery of microresonators

Abstract

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Projects

Living Lasers: H2020 Marie Curie Fellowship 2014 LIVING LASERS

H2020 ERC Starting Grant ABLASE: H2020 ERC Starting Grant ABLASE (Grant Agreement No. 640012)

EU FP7 Marie Curie CIG BioLAS: EU FP7 Marie Curie CIG BioLAS

Datasets

Data underpinning - Lasing in Live Mitotic and Non-Phagocytic Cells by Efficient Delivery of Microresonators

Student theses

Living lasers: lasing from biological and biocompatible soft matter

Cite this