Lasing from fluorescent protein crystals

H.J. Oh; M.C. Gather; J.-J. Song; S.H. Yun

doi:10.1364/OE.22.031411

Lasing from fluorescent protein crystals

H.J. Oh, M.C. Gather, J.-J. Song, S.H. Yun

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28 Citations (Scopus)

Abstract

We investigated fluorescent protein crystals for potential photonic applications, for the first time to our knowledge. Rod-shaped crystals of enhanced green fluorescent protein (EGFP) were synthesized, with diameters of 0.5-2 μm and lengths of 100-200 μm. The crystals exhibit minimal light scattering due to their ordered structure and generate substantially higher fluorescence intensity than EGFP or dye molecules in solutions. The magnitude of concentration quenching in EGFP crystals was measured to be about 7-10 dB. Upon optical pumping at 485 nm, individual EGFP crystals located between dichroic mirrors generated laser emission with a single-mode spectral line at 513 nm. Our results demonstrate the potential of protein crystals as novel optical elements for self-assembled, micro- or nano-lasers and amplifiers in aqueous environment.

Original language	English
Pages (from-to)	31411-31416
Number of pages	6
Journal	Optics Express
Volume	22
Issue number	25
Early online date	11 Dec 2014
DOIs	https://doi.org/10.1364/OE.22.031411
Publication status	Published - 15 Dec 2014

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10.1364/OE.22.031411

EU FP7 Marie Curie CIG BioLAS: EU FP7 Marie Curie CIG BioLAS
Gather, M. C. (PI)
European Commission
1/08/13 → 31/07/17
Project: Standard

Cite this

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title = "Lasing from fluorescent protein crystals",

abstract = "We investigated fluorescent protein crystals for potential photonic applications, for the first time to our knowledge. Rod-shaped crystals of enhanced green fluorescent protein (EGFP) were synthesized, with diameters of 0.5-2 μm and lengths of 100-200 μm. The crystals exhibit minimal light scattering due to their ordered structure and generate substantially higher fluorescence intensity than EGFP or dye molecules in solutions. The magnitude of concentration quenching in EGFP crystals was measured to be about 7-10 dB. Upon optical pumping at 485 nm, individual EGFP crystals located between dichroic mirrors generated laser emission with a single-mode spectral line at 513 nm. Our results demonstrate the potential of protein crystals as novel optical elements for self-assembled, micro- or nano-lasers and amplifiers in aqueous environment.",

author = "H.J. Oh and M.C. Gather and J.-J. Song and S.H. Yun",

note = "This work was funded by the National Science Foundation (ECCS-1101947, EEC-1358296), the Korea National Research Foundation (R31-2008-000-10071-0), the KAIST-MGH Summer Internship Program, and the European Union Marie Curie Career Integration Grant (PCIG12-GA-2012-334407). ",

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doi = "10.1364/OE.22.031411",

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T1 - Lasing from fluorescent protein crystals

AU - Oh, H.J.

AU - Gather, M.C.

AU - Song, J.-J.

AU - Yun, S.H.

N1 - This work was funded by the National Science Foundation (ECCS-1101947, EEC-1358296), the Korea National Research Foundation (R31-2008-000-10071-0), the KAIST-MGH Summer Internship Program, and the European Union Marie Curie Career Integration Grant (PCIG12-GA-2012-334407).

PY - 2014/12/15

Y1 - 2014/12/15

N2 - We investigated fluorescent protein crystals for potential photonic applications, for the first time to our knowledge. Rod-shaped crystals of enhanced green fluorescent protein (EGFP) were synthesized, with diameters of 0.5-2 μm and lengths of 100-200 μm. The crystals exhibit minimal light scattering due to their ordered structure and generate substantially higher fluorescence intensity than EGFP or dye molecules in solutions. The magnitude of concentration quenching in EGFP crystals was measured to be about 7-10 dB. Upon optical pumping at 485 nm, individual EGFP crystals located between dichroic mirrors generated laser emission with a single-mode spectral line at 513 nm. Our results demonstrate the potential of protein crystals as novel optical elements for self-assembled, micro- or nano-lasers and amplifiers in aqueous environment.

AB - We investigated fluorescent protein crystals for potential photonic applications, for the first time to our knowledge. Rod-shaped crystals of enhanced green fluorescent protein (EGFP) were synthesized, with diameters of 0.5-2 μm and lengths of 100-200 μm. The crystals exhibit minimal light scattering due to their ordered structure and generate substantially higher fluorescence intensity than EGFP or dye molecules in solutions. The magnitude of concentration quenching in EGFP crystals was measured to be about 7-10 dB. Upon optical pumping at 485 nm, individual EGFP crystals located between dichroic mirrors generated laser emission with a single-mode spectral line at 513 nm. Our results demonstrate the potential of protein crystals as novel optical elements for self-assembled, micro- or nano-lasers and amplifiers in aqueous environment.

U2 - 10.1364/OE.22.031411

DO - 10.1364/OE.22.031411

M3 - Article

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SN - 1094-4087

VL - 22

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EP - 31416

JO - Optics Express

JF - Optics Express

IS - 25

ER -

Lasing from fluorescent protein crystals

Abstract

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Projects

EU FP7 Marie Curie CIG BioLAS: EU FP7 Marie Curie CIG BioLAS

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