Label-Free Probing of G-Quadruplex Formation by Surface-Enhanced Raman Scattering

Giulia Rusciano*, Anna Chiara De Luca, Giuseppe Pesce, Antonio Sasso, Giorgia Oliviero, Jussara Amato, Nicola Borbone, Stefano D'Errico, Vincenzo Piccialli, Gennaro Piccialli, Luciano Mayol

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

In this work, we establish the use of surface-enhanced Raman scattering (SERS) as a label-free analytical technique for the direct detection of G-quadruplex formation. In particular, we demonstrate that SERS analysis allows the evaluation of the relative stability of G quadruplexes that differ for the number of G tetrads and investigate several structural features of quadruplexes, such as the orientation of glycosidic bonds, the identification of distortions in the sugar-phosphate backbone, and the degree of hydrogen-bond solvation. Herein, the fluctuation of the SERS spectra, due to the specific interaction of vibrational modes with the SERS-active substrate, is quantitatively analyzed before and after quadruplex formation. The results of this study suggest a perpendicular orientation of the quadruplexes (with or without the 3'-tetra end linker) with respect to the silver colloidal surface, which opens new perspectives for the use of SERS as a label-free analytical tool for the study of the binding mode between quadruplexes and their ligands.

Original languageEnglish
Pages (from-to)6849-6855
Number of pages7
JournalAnalytical Chemistry
Volume83
Issue number17
DOIs
Publication statusPublished - 1 Sept 2011

Keywords

  • END-LINKED OLIGONUCLEOTIDES
  • NOVA TELOMERIC DNA
  • SILVER ELECTRODE
  • THROMBIN-BINDING
  • NUCLEIC-ACIDS
  • SPECTROSCOPY
  • SERS
  • GOLD
  • POLYMORPHISM
  • ORIENTATION

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