Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor-2 inhibits type 1 interferon signalling by targeting interferon-stimulated gene factor-3

M. Mutocheluh; L. Hindle; C. Areste; S. A. Chanas; L. M. Butler; K. Lowry; K. Shah; D. J. Evans; D. J. Blackbourn

doi:10.1099/vir.0.034322-0

Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor-2 inhibits type 1 interferon signalling by targeting interferon-stimulated gene factor-3

M. Mutocheluh, L. Hindle, C. Areste, S. A. Chanas, L. M. Butler, K. Lowry, K. Shah, D. J. Evans, D. J. Blackbourn^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) encodes four viral interferon regulatory factors (vIRF-1-4). We investigated the mechanism and consequences of vIRF-2-mediated inhibition of interferon-response element signalling following type 1 interferon (IFN) induction. Western blot and electrophoretic mobility-shift assays identified the interferon-stimulated gene factor-3 (ISGF-3) components STAT1 and IRF-9 as the proximal targets of vIRF-2 activity. The biological significance of vIRF-2 inhibition of ISGF-3 was demonstrated by vIRF-2-mediated rescue of the replication of the IFN-sensitive virus encephalomyocarditis virus. This study provides both a mechanism and evidence for KSHV vIRF-2-mediated suppression of the consequences of type 1 IFN-induced signalling.

Original language	English
Pages (from-to)	2394-2398
Number of pages	5
Journal	Journal of General Virology
Volume	92
DOIs	https://doi.org/10.1099/vir.0.034322-0
Publication status	Published - Oct 2011

Keywords

IMMUNE MODULATION
PROTEIN
IDENTIFICATION
TRANSCRIPTION
PATHWAY
KINASE
CELLS

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10.1099/vir.0.034322-0

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Mutocheluh, M., Hindle, L., Areste, C., Chanas, S. A., Butler, L. M., Lowry, K., Shah, K., Evans, D. J., & Blackbourn, D. J. (2011). Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor-2 inhibits type 1 interferon signalling by targeting interferon-stimulated gene factor-3. Journal of General Virology, 92, 2394-2398. https://doi.org/10.1099/vir.0.034322-0

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title = "Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor-2 inhibits type 1 interferon signalling by targeting interferon-stimulated gene factor-3",

abstract = "Kaposi's sarcoma-associated herpesvirus (KSHV) encodes four viral interferon regulatory factors (vIRF-1-4). We investigated the mechanism and consequences of vIRF-2-mediated inhibition of interferon-response element signalling following type 1 interferon (IFN) induction. Western blot and electrophoretic mobility-shift assays identified the interferon-stimulated gene factor-3 (ISGF-3) components STAT1 and IRF-9 as the proximal targets of vIRF-2 activity. The biological significance of vIRF-2 inhibition of ISGF-3 was demonstrated by vIRF-2-mediated rescue of the replication of the IFN-sensitive virus encephalomyocarditis virus. This study provides both a mechanism and evidence for KSHV vIRF-2-mediated suppression of the consequences of type 1 IFN-induced signalling.",

keywords = "IMMUNE MODULATION, PROTEIN, IDENTIFICATION, TRANSCRIPTION, PATHWAY, KINASE, CELLS",

author = "M. Mutocheluh and L. Hindle and C. Areste and Chanas, {S. A.} and Butler, {L. M.} and K. Lowry and K. Shah and Evans, {D. J.} and Blackbourn, {D. J.}",

year = "2011",

month = oct,

doi = "10.1099/vir.0.034322-0",

language = "English",

volume = "92",

pages = "2394--2398",

journal = "Journal of General Virology",

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Mutocheluh, M, Hindle, L, Areste, C, Chanas, SA, Butler, LM, Lowry, K, Shah, K, Evans, DJ & Blackbourn, DJ 2011, 'Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor-2 inhibits type 1 interferon signalling by targeting interferon-stimulated gene factor-3', Journal of General Virology, vol. 92, pp. 2394-2398. https://doi.org/10.1099/vir.0.034322-0

TY - JOUR

T1 - Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor-2 inhibits type 1 interferon signalling by targeting interferon-stimulated gene factor-3

AU - Mutocheluh, M.

AU - Hindle, L.

AU - Areste, C.

AU - Chanas, S. A.

AU - Butler, L. M.

AU - Lowry, K.

AU - Shah, K.

AU - Evans, D. J.

AU - Blackbourn, D. J.

PY - 2011/10

Y1 - 2011/10

N2 - Kaposi's sarcoma-associated herpesvirus (KSHV) encodes four viral interferon regulatory factors (vIRF-1-4). We investigated the mechanism and consequences of vIRF-2-mediated inhibition of interferon-response element signalling following type 1 interferon (IFN) induction. Western blot and electrophoretic mobility-shift assays identified the interferon-stimulated gene factor-3 (ISGF-3) components STAT1 and IRF-9 as the proximal targets of vIRF-2 activity. The biological significance of vIRF-2 inhibition of ISGF-3 was demonstrated by vIRF-2-mediated rescue of the replication of the IFN-sensitive virus encephalomyocarditis virus. This study provides both a mechanism and evidence for KSHV vIRF-2-mediated suppression of the consequences of type 1 IFN-induced signalling.

AB - Kaposi's sarcoma-associated herpesvirus (KSHV) encodes four viral interferon regulatory factors (vIRF-1-4). We investigated the mechanism and consequences of vIRF-2-mediated inhibition of interferon-response element signalling following type 1 interferon (IFN) induction. Western blot and electrophoretic mobility-shift assays identified the interferon-stimulated gene factor-3 (ISGF-3) components STAT1 and IRF-9 as the proximal targets of vIRF-2 activity. The biological significance of vIRF-2 inhibition of ISGF-3 was demonstrated by vIRF-2-mediated rescue of the replication of the IFN-sensitive virus encephalomyocarditis virus. This study provides both a mechanism and evidence for KSHV vIRF-2-mediated suppression of the consequences of type 1 IFN-induced signalling.

KW - IMMUNE MODULATION

KW - PROTEIN

KW - IDENTIFICATION

KW - TRANSCRIPTION

KW - PATHWAY

KW - KINASE

KW - CELLS

U2 - 10.1099/vir.0.034322-0

DO - 10.1099/vir.0.034322-0

M3 - Article

SN - 0022-1317

VL - 92

SP - 2394

EP - 2398

JO - Journal of General Virology

JF - Journal of General Virology

ER -

Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor-2 inhibits type 1 interferon signalling by targeting interferon-stimulated gene factor-3

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Keywords

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