Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor-2 inhibits type 1 interferon signalling by targeting interferon-stimulated gene factor-3

M. Mutocheluh, L. Hindle, C. Areste, S. A. Chanas, L. M. Butler, K. Lowry, K. Shah, D. J. Evans, D. J. Blackbourn*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) encodes four viral interferon regulatory factors (vIRF-1-4). We investigated the mechanism and consequences of vIRF-2-mediated inhibition of interferon-response element signalling following type 1 interferon (IFN) induction. Western blot and electrophoretic mobility-shift assays identified the interferon-stimulated gene factor-3 (ISGF-3) components STAT1 and IRF-9 as the proximal targets of vIRF-2 activity. The biological significance of vIRF-2 inhibition of ISGF-3 was demonstrated by vIRF-2-mediated rescue of the replication of the IFN-sensitive virus encephalomyocarditis virus. This study provides both a mechanism and evidence for KSHV vIRF-2-mediated suppression of the consequences of type 1 IFN-induced signalling.

Original languageEnglish
Pages (from-to)2394-2398
Number of pages5
JournalJournal of General Virology
Volume92
DOIs
Publication statusPublished - Oct 2011

Keywords

  • IMMUNE MODULATION
  • PROTEIN
  • IDENTIFICATION
  • TRANSCRIPTION
  • PATHWAY
  • KINASE
  • CELLS

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