Isolation of lactobacilli from sow milk and evaluation of their probiotic potential

Rocio Martin; Susana Delgado; Antonio Maldonado; Esther Jimenez; Monica Olivares; Leonides Fernandez; Odon J. Sobrino; Juan M. Rodriguez

doi:10.1017/S0022029909990124

Isolation of lactobacilli from sow milk and evaluation of their probiotic potential

Rocio Martin, Susana Delgado, Antonio Maldonado, Esther Jimenez, Monica Olivares, Leonides Fernandez, Odon J. Sobrino, Juan M. Rodriguez^*

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Sow milk protects the piglet against infectious diseases through a variety of mechanisms. In this study, the presence of potentially probiotic lactic acid bacteria in this biological fluid was investigated. Milk samples were obtained from 8 sows and a total of 19 rod-shaped isolates were selected for identification and assessment of their probiotic potential. RAPID profiling revealed the existence of 8 different genetic profiles among them. One representative of each profile was selected for further characterization and they were identified as Lactobacillus reuteri, Lb. salivarius, Lb. plantarum, Lb. paraplantarum, Lb. brevis and Weissella paramesenteroides. Then, their probiotic potential was evaluated through different assays, including survival in conditions simulating those existing in the gastrointestinal tract, production of antimicrobial compounds, adherence to intestinal mucin, production of biogenic amines, degradation of mucin, and pattern of antibiotic sensitivity. Three strains, Lb. reuteri CR20 (a reuterin-producing strain), Lb. salivarius CELA2 (a bacteriocin-producing strain) and Lb. paraplantarum CLB7 displayed the highest probiotic potential.

Original language	English
Pages (from-to)	418-425
Number of pages	8
Journal	Journal of dairy research
Volume	76
Issue number	4
DOIs	https://doi.org/10.1017/S0022029909990124
Publication status	Published - Nov 2009

Keywords

Lactobacillus
sow
pig
milk
probiotics
LACTIC-ACID BACTERIA
GASTROINTESTINAL-TRACT
MONITOR CHANGES
BREAST-MILK
INFANT GUT
IN-VITRO
MICROBIOTA
SURVIVAL
REUTERI
PIGS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1017/S0022029909990124

Cite this

@article{1727a65c37984fcdbe625708d644b696,

title = "Isolation of lactobacilli from sow milk and evaluation of their probiotic potential",

abstract = "Sow milk protects the piglet against infectious diseases through a variety of mechanisms. In this study, the presence of potentially probiotic lactic acid bacteria in this biological fluid was investigated. Milk samples were obtained from 8 sows and a total of 19 rod-shaped isolates were selected for identification and assessment of their probiotic potential. RAPID profiling revealed the existence of 8 different genetic profiles among them. One representative of each profile was selected for further characterization and they were identified as Lactobacillus reuteri, Lb. salivarius, Lb. plantarum, Lb. paraplantarum, Lb. brevis and Weissella paramesenteroides. Then, their probiotic potential was evaluated through different assays, including survival in conditions simulating those existing in the gastrointestinal tract, production of antimicrobial compounds, adherence to intestinal mucin, production of biogenic amines, degradation of mucin, and pattern of antibiotic sensitivity. Three strains, Lb. reuteri CR20 (a reuterin-producing strain), Lb. salivarius CELA2 (a bacteriocin-producing strain) and Lb. paraplantarum CLB7 displayed the highest probiotic potential.",

keywords = "Lactobacillus, sow, pig, milk, probiotics, LACTIC-ACID BACTERIA, GASTROINTESTINAL-TRACT, MONITOR CHANGES, BREAST-MILK, INFANT GUT, IN-VITRO, MICROBIOTA, SURVIVAL, REUTERI, PIGS",

author = "Rocio Martin and Susana Delgado and Antonio Maldonado and Esther Jimenez and Monica Olivares and Leonides Fernandez and Sobrino, {Odon J.} and Rodriguez, {Juan M.}",

year = "2009",

month = nov,

doi = "10.1017/S0022029909990124",

language = "English",

volume = "76",

pages = "418--425",

journal = "Journal of dairy research",

issn = "0022-0299",

publisher = "Cambridge University Press",

number = "4",

}

TY - JOUR

T1 - Isolation of lactobacilli from sow milk and evaluation of their probiotic potential

AU - Martin, Rocio

AU - Delgado, Susana

AU - Maldonado, Antonio

AU - Jimenez, Esther

AU - Olivares, Monica

AU - Fernandez, Leonides

AU - Sobrino, Odon J.

AU - Rodriguez, Juan M.

PY - 2009/11

Y1 - 2009/11

N2 - Sow milk protects the piglet against infectious diseases through a variety of mechanisms. In this study, the presence of potentially probiotic lactic acid bacteria in this biological fluid was investigated. Milk samples were obtained from 8 sows and a total of 19 rod-shaped isolates were selected for identification and assessment of their probiotic potential. RAPID profiling revealed the existence of 8 different genetic profiles among them. One representative of each profile was selected for further characterization and they were identified as Lactobacillus reuteri, Lb. salivarius, Lb. plantarum, Lb. paraplantarum, Lb. brevis and Weissella paramesenteroides. Then, their probiotic potential was evaluated through different assays, including survival in conditions simulating those existing in the gastrointestinal tract, production of antimicrobial compounds, adherence to intestinal mucin, production of biogenic amines, degradation of mucin, and pattern of antibiotic sensitivity. Three strains, Lb. reuteri CR20 (a reuterin-producing strain), Lb. salivarius CELA2 (a bacteriocin-producing strain) and Lb. paraplantarum CLB7 displayed the highest probiotic potential.

AB - Sow milk protects the piglet against infectious diseases through a variety of mechanisms. In this study, the presence of potentially probiotic lactic acid bacteria in this biological fluid was investigated. Milk samples were obtained from 8 sows and a total of 19 rod-shaped isolates were selected for identification and assessment of their probiotic potential. RAPID profiling revealed the existence of 8 different genetic profiles among them. One representative of each profile was selected for further characterization and they were identified as Lactobacillus reuteri, Lb. salivarius, Lb. plantarum, Lb. paraplantarum, Lb. brevis and Weissella paramesenteroides. Then, their probiotic potential was evaluated through different assays, including survival in conditions simulating those existing in the gastrointestinal tract, production of antimicrobial compounds, adherence to intestinal mucin, production of biogenic amines, degradation of mucin, and pattern of antibiotic sensitivity. Three strains, Lb. reuteri CR20 (a reuterin-producing strain), Lb. salivarius CELA2 (a bacteriocin-producing strain) and Lb. paraplantarum CLB7 displayed the highest probiotic potential.

KW - Lactobacillus

KW - sow

KW - pig

KW - milk

KW - probiotics

KW - LACTIC-ACID BACTERIA

KW - GASTROINTESTINAL-TRACT

KW - MONITOR CHANGES

KW - BREAST-MILK

KW - INFANT GUT

KW - IN-VITRO

KW - MICROBIOTA

KW - SURVIVAL

KW - REUTERI

KW - PIGS

U2 - 10.1017/S0022029909990124

DO - 10.1017/S0022029909990124

M3 - Article

SN - 0022-0299

VL - 76

SP - 418

EP - 425

JO - Journal of dairy research

JF - Journal of dairy research

IS - 4

ER -

Isolation of lactobacilli from sow milk and evaluation of their probiotic potential

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this