Isolation and characterization of microsatellite loci in Senecio

G Liu; MJ Hegarty; KJ Edwards; SJ Hiscock; Richard John Abbott

doi:10.1111/j.1477-8286.2004.00753.x

Isolation and characterization of microsatellite loci in Senecio

G Liu, MJ Hegarty, KJ Edwards, SJ Hiscock, Richard John Abbott

Research output: Other contribution

Abstract

Interspecific hybridization has resulted in the recent origin of several hybrid Senecio taxa at diploid, tetraploid and hexaploid levels. As part of research aimed at constructing and comparing genomic maps of each of these taxa and their parents, we have isolated micro-satellite loci from genomic DNA libraries of S. vulgaris and S. squalidus. Primers of 35 loci amplified microsatellites resolved in agarose gels from one or more of S. vulgaris, S. squalidus, S. aethnensis and S. chrysanthemifolius. Approximately 71% of primers amplified a product in all four species. A survey of microsatellite variation in S. chrysanthemifolius over a subset of 14 loci resolved 2-11 alleles per locus in polyacrylamide gels with expected heterozygosity (HE) ranging from 0.26 to 0.87.

Original language	English
Volume	4
DOIs	https://doi.org/10.1111/j.1477-8286.2004.00753.x
Publication status	Published - Dec 2004

Keywords

hybridization
microsatellite
Senecio
speciation
SSR
RADIATE GROUNDSEL
HYBRIDIZATION
ORIGINS

Access to Document

10.1111/j.1477-8286.2004.00753.x

Cite this

@misc{329df4740c4d421087d0d472e093d0e0,

title = "Isolation and characterization of microsatellite loci in Senecio",

abstract = "Interspecific hybridization has resulted in the recent origin of several hybrid Senecio taxa at diploid, tetraploid and hexaploid levels. As part of research aimed at constructing and comparing genomic maps of each of these taxa and their parents, we have isolated micro-satellite loci from genomic DNA libraries of S. vulgaris and S. squalidus. Primers of 35 loci amplified microsatellites resolved in agarose gels from one or more of S. vulgaris, S. squalidus, S. aethnensis and S. chrysanthemifolius. Approximately 71% of primers amplified a product in all four species. A survey of microsatellite variation in S. chrysanthemifolius over a subset of 14 loci resolved 2-11 alleles per locus in polyacrylamide gels with expected heterozygosity (HE) ranging from 0.26 to 0.87.",

keywords = "hybridization, microsatellite, Senecio, speciation, SSR, RADIATE GROUNDSEL, HYBRIDIZATION, ORIGINS",

author = "G Liu and MJ Hegarty and KJ Edwards and SJ Hiscock and Abbott, {Richard John}",

note = "<i>Molecular Ecology Notes</i>",

year = "2004",

month = dec,

doi = "10.1111/j.1477-8286.2004.00753.x",

language = "English",

volume = "4",

type = "Other",

}

TY - GEN

T1 - Isolation and characterization of microsatellite loci in Senecio

AU - Liu, G

AU - Hegarty, MJ

AU - Edwards, KJ

AU - Hiscock, SJ

AU - Abbott, Richard John

N1 - <i>Molecular Ecology Notes</i>

PY - 2004/12

Y1 - 2004/12

N2 - Interspecific hybridization has resulted in the recent origin of several hybrid Senecio taxa at diploid, tetraploid and hexaploid levels. As part of research aimed at constructing and comparing genomic maps of each of these taxa and their parents, we have isolated micro-satellite loci from genomic DNA libraries of S. vulgaris and S. squalidus. Primers of 35 loci amplified microsatellites resolved in agarose gels from one or more of S. vulgaris, S. squalidus, S. aethnensis and S. chrysanthemifolius. Approximately 71% of primers amplified a product in all four species. A survey of microsatellite variation in S. chrysanthemifolius over a subset of 14 loci resolved 2-11 alleles per locus in polyacrylamide gels with expected heterozygosity (HE) ranging from 0.26 to 0.87.

AB - Interspecific hybridization has resulted in the recent origin of several hybrid Senecio taxa at diploid, tetraploid and hexaploid levels. As part of research aimed at constructing and comparing genomic maps of each of these taxa and their parents, we have isolated micro-satellite loci from genomic DNA libraries of S. vulgaris and S. squalidus. Primers of 35 loci amplified microsatellites resolved in agarose gels from one or more of S. vulgaris, S. squalidus, S. aethnensis and S. chrysanthemifolius. Approximately 71% of primers amplified a product in all four species. A survey of microsatellite variation in S. chrysanthemifolius over a subset of 14 loci resolved 2-11 alleles per locus in polyacrylamide gels with expected heterozygosity (HE) ranging from 0.26 to 0.87.

KW - hybridization

KW - microsatellite

KW - Senecio

KW - speciation

KW - SSR

KW - RADIATE GROUNDSEL

KW - HYBRIDIZATION

KW - ORIGINS

UR - http://www.scopus.com/inward/record.url?scp=9644300855&partnerID=8YFLogxK

U2 - 10.1111/j.1477-8286.2004.00753.x

DO - 10.1111/j.1477-8286.2004.00753.x

M3 - Other contribution

VL - 4

ER -

Isolation and characterization of microsatellite loci in Senecio

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this