Isolation and characterization of microsatellite loci in Senecio

G Liu, MJ Hegarty, KJ Edwards, SJ Hiscock, Richard John Abbott

Research output: Other contribution

10 Citations (Scopus)

Abstract

Interspecific hybridization has resulted in the recent origin of several hybrid Senecio taxa at diploid, tetraploid and hexaploid levels. As part of research aimed at constructing and comparing genomic maps of each of these taxa and their parents, we have isolated micro-satellite loci from genomic DNA libraries of S. vulgaris and S. squalidus. Primers of 35 loci amplified microsatellites resolved in agarose gels from one or more of S. vulgaris, S. squalidus, S. aethnensis and S. chrysanthemifolius. Approximately 71% of primers amplified a product in all four species. A survey of microsatellite variation in S. chrysanthemifolius over a subset of 14 loci resolved 2-11 alleles per locus in polyacrylamide gels with expected heterozygosity (HE) ranging from 0.26 to 0.87.

Original languageEnglish
Volume4
DOIs
Publication statusPublished - Dec 2004

Keywords

  • hybridization
  • microsatellite
  • Senecio
  • speciation
  • SSR
  • RADIATE GROUNDSEL
  • HYBRIDIZATION
  • ORIGINS

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