Isolation and characterization of a putative collagen gene from the potato cyst nematode Globodera pallida

JT Jones*, RH Curtis, PJ Wightman, PR Burrows

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

A cDNA clone encoding a full length putative collagen has been isolated in a screen of a mixed stage Globodera pallida expression library. Comparison of the deduced amino acid sequence of this molecule with other collagens suggests it is a cuticular collagen and a member of the col-8 subfamily of collagen genes. Northern blots show the gene is expressed specifically in gravid, adult females of the parasite as compared to second (invasive) stage juveniles and virgin females. Preliminary immunocytochemical studies indicate this collagen is present in areas other than the cuticle; these findings and the potential functional role of this collagen are discussed.

Original languageEnglish
Pages (from-to)581-588
Number of pages8
JournalParasitology
Volume113
Publication statusPublished - Dec 1996

Keywords

  • nematode
  • collagen
  • Globodera pallida
  • DNA
  • CAENORHABDITIS-ELEGANS
  • MELOIDOGYNE-INCOGNITA
  • HAEMONCHUS-CONTORTUS
  • PARASITIC NEMATODES
  • ASCARIS-SUUM
  • CUTICLE
  • EXPRESSION
  • SEQUENCES
  • PROTEIN

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