Isolation and characterisation of polymorphic microsatellites in the Trinidadian guppy (Poecilia reticulata)

SA Becher; ST Russell; Anne Elizabeth Magurran

Isolation and characterisation of polymorphic microsatellites in the Trinidadian guppy (Poecilia reticulata)

SA Becher, ST Russell, Anne Elizabeth Magurran

Research output: Other contribution

Abstract

We isolated microsatellite loci for the Trinidadian guppy Poecilia reticulata from the Oropuche and Caroni drainages in Trinidad, using a modified enrichment protocol that provided 93% enriched libraries. The eight loci presented here are polymorphic, and have between seven and 18 alleles in 33-48 individuals initially screened. Observed heterozygosities in this sample range from 0.28 to 0.88. These loci significantly increase the number of polymorphic microsatellite markers available for paternity and population analysis in this species.

Original language	English
Volume	2
Publication status	Published - Dec 2002

Keywords

enrichment
microsatellites
paternity analysis
Poeciliidae
population
SELECTION

Cite this

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title = "Isolation and characterisation of polymorphic microsatellites in the Trinidadian guppy (Poecilia reticulata)",

abstract = "We isolated microsatellite loci for the Trinidadian guppy Poecilia reticulata from the Oropuche and Caroni drainages in Trinidad, using a modified enrichment protocol that provided 93\% enriched libraries. The eight loci presented here are polymorphic, and have between seven and 18 alleles in 33-48 individuals initially screened. Observed heterozygosities in this sample range from 0.28 to 0.88. These loci significantly increase the number of polymorphic microsatellite markers available for paternity and population analysis in this species.",

keywords = "enrichment, microsatellites, paternity analysis, Poeciliidae, population, SELECTION",

author = "SA Becher and ST Russell and Magurran, \{Anne Elizabeth\}",

note = "Mol Ecol Notes",

year = "2002",

month = dec,

language = "English",

volume = "2",

type = "Other",

}

TY - GEN

T1 - Isolation and characterisation of polymorphic microsatellites in the Trinidadian guppy (Poecilia reticulata)

AU - Becher, SA

AU - Russell, ST

AU - Magurran, Anne Elizabeth

N1 - Mol Ecol Notes

PY - 2002/12

Y1 - 2002/12

N2 - We isolated microsatellite loci for the Trinidadian guppy Poecilia reticulata from the Oropuche and Caroni drainages in Trinidad, using a modified enrichment protocol that provided 93% enriched libraries. The eight loci presented here are polymorphic, and have between seven and 18 alleles in 33-48 individuals initially screened. Observed heterozygosities in this sample range from 0.28 to 0.88. These loci significantly increase the number of polymorphic microsatellite markers available for paternity and population analysis in this species.

AB - We isolated microsatellite loci for the Trinidadian guppy Poecilia reticulata from the Oropuche and Caroni drainages in Trinidad, using a modified enrichment protocol that provided 93% enriched libraries. The eight loci presented here are polymorphic, and have between seven and 18 alleles in 33-48 individuals initially screened. Observed heterozygosities in this sample range from 0.28 to 0.88. These loci significantly increase the number of polymorphic microsatellite markers available for paternity and population analysis in this species.

KW - enrichment

KW - microsatellites

KW - paternity analysis

KW - Poeciliidae

KW - population

KW - SELECTION

UR - https://www.scopus.com/pages/publications/0036899874

M3 - Other contribution

VL - 2

ER -

Isolation and characterisation of polymorphic microsatellites in the Trinidadian guppy (Poecilia reticulata)

Abstract

Keywords

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