Investigation of the reliability of 24 h urine excretion as a biomarker of isoflavone exposure over time and over a wide range of isoflavone intakes

Margaret Renwick Ritchie, M S Morton, A M Thompson, N Deighton, A Blake, J H Cummings, Christopher Michael Steel

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Objective: To study the variation in genistein+daidzein intake over a 6-month period and test the reliability of 24 h urinary isoflavones as a biomarker of exposure over time.

Design: Dietary genistein+daidzein intake was assessed at various time points throughout six months in 15 healthy subjects. Group 1 (n=8) followed nonsupplemented diets and Group 2 (n=7) took a 35 mg/d isoflavone supplement for 3 months and each subject provided a 24 h urine collection, validated with para-aminobenzoic acid, during weeks 7, 15 and 19. Urine was analysed for genistein and daidzein using LC-MS.

Results: Isoflavone intake in Groups 1 and 2 ranged from 0.00 to 1.1 mg/d and 0.1 to 53.1 mg/d, respectively. Urine excretion for both groups ranged from 0.20 to 9.56 mg/d. The relationship between 24 h excretion and isoflavone intake is y=0.44x +/-0.03(standard deviation) +1.57; r=0.89, P<0.001.

Conclusion: The 24 h urinary isoflavones can be used as biomarkers of isoflavone exposure over time.

Original languageEnglish
Pages (from-to)1286-1289
Number of pages4
JournalEuropean Journal of Clinical Nutrition
Volume58
Issue number9
DOIs
Publication statusPublished - Sept 2004

Keywords

  • isoflavones
  • biomarker
  • urine
  • time course
  • dietary supplementation
  • GENISTEIN
  • ESTROGENS
  • DAIDZEIN
  • PLASMA
  • CANCER
  • WOMEN

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