Investigation of refractive index dynamics during in vitro embryo development using off-axis digital holographic microscopy

George Dwapanyin, Darren Chow, Tiffany Tan, Nicolas Dubost, Josephine Morizet, Kylie Dunning, Kishan Dholakia*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

Embryo quality is a crucial factor affecting live birth outcomes. However, an accurate diagnostic for embryo quality remains elusive in the in vitro fertilization clinic. Determining physical parameters of the embryo may offer key information for this purpose. Here, we demonstrate that digital holographic microscopy (DHM) can rapidly and non-invasively assess the refractive index of mouse embryos. Murine embryos were cultured in either low- or high-lipid containing media and digital holograms recorded at various stages of development. The phase of the recorded hologram was numerically retrieved, from which the refractive index of the embryo was calculated. We showed that DHM can detect spatio-temporal changes in refractive index during embryo development that are reflective of its lipid content. As accumulation of intracellular lipid is known to compromise embryo health, DHM may prove beneficial in developing an accurate, non-invasive, multimodal diagnostic.
Original languageEnglish
Pages (from-to)3327-3342
Number of pages16
JournalBiomedical Optics Express
Volume14
Issue number7
Early online date13 Jun 2023
DOIs
Publication statusPublished - 1 Jul 2023

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