Investigation of a choline phosphate synthesis pathway in Streptococcus pneumoniae: Evidence for choline phosphate cytidylyltransferase activity

Gail C. Whiting; Stephen H. Gillespie

doi:10.1016/0378-1097(96)00328-X

Investigation of a choline phosphate synthesis pathway in Streptococcus pneumoniae: Evidence for choline phosphate cytidylyltransferase activity

Gail C. Whiting, Stephen H. Gillespie^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

In this study we have demonstrated the activity of a choline phosphate cytidylyltransferase in cell free extracts of Streptococcus pneumoniae. Southern blot analysis of restricted S. pneumoniae genomic DNA probed with the gene coding for the choline phosphate cytidylyltransferase of Saccharomyces cerevisiae demonstrated that there is homology between the S. cerevisiae cct gene and genomic DNA of S. pneumoniae. We believe that this enzyme is involved in the biosynthesis of the choline containing cell wall antigens, teichoic acid and lipoteichoic acid, catalysing the activation of choline phosphate to CDP-choline which is then incorporated into the polysaccharide moiety.

Original language	English
Pages (from-to)	279-284
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	143
Issue number	2-3
DOIs	https://doi.org/10.1016/0378-1097(96)00328-X
Publication status	Published - 1 Oct 1996

Keywords

Choline metabolism
Cytidylyltransferase
Lipoteichoic acid
Streptococcus pneumoniae
Teichoic acid

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10.1016/0378-1097(96)00328-X

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title = "Investigation of a choline phosphate synthesis pathway in Streptococcus pneumoniae: Evidence for choline phosphate cytidylyltransferase activity",

abstract = "In this study we have demonstrated the activity of a choline phosphate cytidylyltransferase in cell free extracts of Streptococcus pneumoniae. Southern blot analysis of restricted S. pneumoniae genomic DNA probed with the gene coding for the choline phosphate cytidylyltransferase of Saccharomyces cerevisiae demonstrated that there is homology between the S. cerevisiae cct gene and genomic DNA of S. pneumoniae. We believe that this enzyme is involved in the biosynthesis of the choline containing cell wall antigens, teichoic acid and lipoteichoic acid, catalysing the activation of choline phosphate to CDP-choline which is then incorporated into the polysaccharide moiety.",

keywords = "Choline metabolism, Cytidylyltransferase, Lipoteichoic acid, Streptococcus pneumoniae, Teichoic acid",

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T1 - Investigation of a choline phosphate synthesis pathway in Streptococcus pneumoniae

T2 - Evidence for choline phosphate cytidylyltransferase activity

AU - Whiting, Gail C.

AU - Gillespie, Stephen H.

PY - 1996/10/1

Y1 - 1996/10/1

N2 - In this study we have demonstrated the activity of a choline phosphate cytidylyltransferase in cell free extracts of Streptococcus pneumoniae. Southern blot analysis of restricted S. pneumoniae genomic DNA probed with the gene coding for the choline phosphate cytidylyltransferase of Saccharomyces cerevisiae demonstrated that there is homology between the S. cerevisiae cct gene and genomic DNA of S. pneumoniae. We believe that this enzyme is involved in the biosynthesis of the choline containing cell wall antigens, teichoic acid and lipoteichoic acid, catalysing the activation of choline phosphate to CDP-choline which is then incorporated into the polysaccharide moiety.

AB - In this study we have demonstrated the activity of a choline phosphate cytidylyltransferase in cell free extracts of Streptococcus pneumoniae. Southern blot analysis of restricted S. pneumoniae genomic DNA probed with the gene coding for the choline phosphate cytidylyltransferase of Saccharomyces cerevisiae demonstrated that there is homology between the S. cerevisiae cct gene and genomic DNA of S. pneumoniae. We believe that this enzyme is involved in the biosynthesis of the choline containing cell wall antigens, teichoic acid and lipoteichoic acid, catalysing the activation of choline phosphate to CDP-choline which is then incorporated into the polysaccharide moiety.

KW - Choline metabolism

KW - Cytidylyltransferase

KW - Lipoteichoic acid

KW - Streptococcus pneumoniae

KW - Teichoic acid

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AN - SCOPUS:0030271444

SN - 0378-1097

VL - 143

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JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2-3

ER -

Investigation of a choline phosphate synthesis pathway in Streptococcus pneumoniae: Evidence for choline phosphate cytidylyltransferase activity

Abstract

Keywords

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