Intersubunit allosteric communication mediated by a conserved loop in the MCM helicase

Elizabeth R. Barry, Janet E. Lovett, Alessandro Costa, Susan M. Lea, Stephen D. Bell*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

The minichromosome maintenance (MCM) helicase is the presumptive replicative helicase in archaea and eukaryotes. The archaeal homomultimeric MCM has a two-tier structure. One tier contains the AAA+ motor domains of the proteins, and these are the minimal functional helicase domains. The second tier is formed by the N-terminal domains. These domains are not essential for MCM helicase activity but act to enhance the processivity of the helicase. We reveal that a conserved loop facilitates communication between processivity and motor tiers. Interestingly, this allostery seems to be mediated by interactions between, rather than within, individual protomers in the MCM ring.

Original languageEnglish
Pages (from-to)1051-1056
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number4
DOIs
Publication statusPublished - 27 Jan 2009

Keywords

  • archaea
  • DNA replication
  • AAA plus protein
  • Sulfolobus
  • REPLICATIVE HEXAMERIC HELICASE
  • ARCHAEAL MCM
  • DNA
  • MECHANISM
  • PROTEIN
  • COMPLEX
  • TRANSLOCATION
  • DOMAINS

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