Interaction between echovirus 7 and its receptor, decay-accelerating factor (CD55): Evidence for a secondary cellular factor in A-particle formation

RM Powell, T Ward, DJ Evans, JW Almond

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70 Citations (Scopus)

Abstract

Soluble forms of decay-accelerating factor (DAF) (CD55), the receptor for echovirus 7, were synthesized in the yeast Pichia pastoris. Purified recombinant protein containing SCR domains 2, 3, and 4, but lacking the serine/threonine rich region, was shown to block infection of susceptible cells by echovirus 7. In contrast to the situation with poliovirus and its receptor, the neutralization of echovirus 7 by soluble DAF was completely reversible and did not lead to the formation of 135S A-particles, Binding of virus to susceptible cells, by contrast, did lead to the formation of A particles, mainly from virus that had been internalized, The data suggest that a secondary factor(s) may contribute to A-particle formation and uncoating of echovirus 7.

Original languageEnglish
Pages (from-to)9306-9312
Number of pages7
JournalJournal of Virology
Volume71
Issue number12
Publication statusPublished - Dec 1997

Keywords

  • COMPLEMENT REGULATORY PROTEIN
  • HELA-CELLS
  • HUMAN RHINOVIRUS
  • MEASLES-VIRUS
  • POLIOVIRUS
  • MEMBRANE
  • COFACTOR
  • NEUTRALIZATION
  • DOMAINS
  • BINDING

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