Integrated single- and two-photon light sheet microscopy using accelerating beams

Peeter Piksarv; Dominik Marti; Tuan Le; Angelika Unterhuber; Lindsey Helen Forbes; Melissa Renee Andrews; Andreas Stingl; Wolfgang Drexler; Peter E. Andersen; Kishan Dholakia

doi:10.1038/s41598-017-01543-4

Integrated single- and two-photon light sheet microscopy using accelerating beams

Peeter Piksarv, Dominik Marti, Tuan Le, Angelika Unterhuber, Lindsey Helen Forbes, Melissa Renee Andrews, Andreas Stingl, Wolfgang Drexler, Peter E. Andersen, Kishan Dholakia

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Abstract

We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.

Original language	English
Article number	1435
Journal	Scientific Reports
Volume	7
DOIs	https://doi.org/10.1038/s41598-017-01543-4
Publication status	Published - 3 May 2017

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Cite this

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title = "Integrated single- and two-photon light sheet microscopy using accelerating beams",

abstract = "We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.",

author = "Peeter Piksarv and Dominik Marti and Tuan Le and Angelika Unterhuber and Forbes, {Lindsey Helen} and Andrews, {Melissa Renee} and Andreas Stingl and Wolfgang Drexler and Andersen, {Peter E.} and Kishan Dholakia",

note = "The authors thank the UK Engineering and Physical Sciences Research Council (grant EP/J01771/X) and the European Union project FAMOS (FP7 ICT, contract no. 317744) for funding. P.P. acknowledges funding by the Estonian Research Council (grants PUTJD8 and PUT369).",

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doi = "10.1038/s41598-017-01543-4",

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T1 - Integrated single- and two-photon light sheet microscopy using accelerating beams

AU - Piksarv, Peeter

AU - Marti, Dominik

AU - Le, Tuan

AU - Unterhuber, Angelika

AU - Forbes, Lindsey Helen

AU - Andrews, Melissa Renee

AU - Stingl, Andreas

AU - Drexler, Wolfgang

AU - Andersen, Peter E.

AU - Dholakia, Kishan

N1 - The authors thank the UK Engineering and Physical Sciences Research Council (grant EP/J01771/X) and the European Union project FAMOS (FP7 ICT, contract no. 317744) for funding. P.P. acknowledges funding by the Estonian Research Council (grants PUTJD8 and PUT369).

PY - 2017/5/3

Y1 - 2017/5/3

N2 - We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.

AB - We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.

UR - https://www.nature.com/articles/s41598-017-01543-4#supplementary-information

U2 - 10.1038/s41598-017-01543-4

DO - 10.1038/s41598-017-01543-4

M3 - Article

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

M1 - 1435

ER -

Integrated single- and two-photon light sheet microscopy using accelerating beams

Abstract

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EU FP7 FAMOS: Functional Anatomical Molecular Optical Screening

Challening the Limits of Photonics: stru: Challenging the Limits of Photonics: Structured Light