Integrated holographic system for all-optical manipulation of developing embryos

Maria Leilani Torres, Maciej Antkowiak, Hana Cizmarova, David Ellard Keith Ferrier, Kishan Dholakia, Frank J Gunn-Moore

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)
3 Downloads (Pure)


We demonstrate a system for the combined optical injection and trapping of developing embryos. A Ti:sapphire femtosecond laser in tandem with a spatial light modulator, is used to perform fast and accurate beam-steering and multiplexing. We show successful intracellular delivery of a range of impermeable molecules into individual blastomeres of the annelid Pomatoceros lamarckii embryo by optoinjection, even when the embryo is still enclosed in a chorion. We also demonstrate the ability of the femtosecond laser optoinjection to deliver materials into inner layers of cells in a well-developed embryo. By switching to the continuous wave mode of the Ti:sapphire laser, the same system can be employed to optically trap and orient the 60 μm sized P. lamarckii embryo whilst maintaining its viability. Hence, a complete all-optical manipulation platform is demonstrated paving the way towards single-cell genetic modification and cell lineage mapping in emerging developmental biology model species.
Original languageEnglish
Pages (from-to)1564-1575
Number of pages11
JournalBiomedical Optics Express
Issue number6
Publication statusPublished - 1 Jun 2011


  • Multiphoton processes
  • Lasers
  • Ultrafast lasers


Dive into the research topics of 'Integrated holographic system for all-optical manipulation of developing embryos'. Together they form a unique fingerprint.

Cite this