@inproceedings{dd207b165738460e949f925928f0f59a,
title = "Integrated 3D macro-trapping and light-sheet imaging system",
abstract = "Biological research requires high-speed and low-damage imaging techniques for live specimens in areas such as development study in embryos. Light sheet microscopy provides fast imaging speed whilst keeps the photo-damage and photo-blenching to minimum. Conventional sample embedding methods in light sheet imaging involves using agent such as agarose which potentially affects the behavior and the develop pattern of the specimens. Here we demonstrate integrating dual-beam trapping method into light sheet imaging system to confine and translate the specimen whilst light sheet images are taken. Tobacco plant cells as well as Spirobranchus lamarcki larva were trapped solely with optical force and sectional images were acquired. This now approach has the potential to extend the applications of light sheet imaging significantly.",
keywords = "counter-propagating trap, dual-beam trap, Light sheet imaging, openSPIM, optical trapping, Spirobranchus lamarcki larva.",
author = "Zhengyi Yang and Peeter Piksarv and Ferrier, {David E.K.} and Gunn-Moore, {Frank J.} and Kishan Dholakia",
note = "Publisher Copyright: {\textcopyright} 2015 SPIE.; Optical Trapping and Optical Micromanipulation XII ; Conference date: 09-08-2015 Through 12-08-2015",
year = "2015",
doi = "10.1117/12.2186399",
language = "English",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "SPIE",
editor = "Spalding, {Gabriel C.} and Kishan Dholakia and Kishan Dholakia and Spalding, {Gabriel C.}",
booktitle = "Optical Trapping and Optical Micromanipulation XII",
address = "United States",
}