Instability of CHO chromosomes containing interstial telomeric sequences originating from the Chinese hamster chromosome 10

P Slijepcevic; Y Xiao; AT Natarajan; Peter Edward Bryant

Instability of CHO chromosomes containing interstial telomeric sequences originating from the Chinese hamster chromosome 10

P Slijepcevic, Y Xiao, AT Natarajan, Peter Edward Bryant

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

We identified a marker chromosome in the CHO K1 cell line containing amplifications of interstitial telomeric sequences originating from Chinese hamster chromosome 10. Analysis of the progression of this chromosome in two subclones of CHO K1 revealed sensitivity of one amplicon to chromosome breakage, resulting in telomere function at the break site. In addition, two more marker chromosomes, both containing amplifications of interstitial telomeric sequences from chromosome 10, were formed during karyotypic evolution of the CHO K1 subclones. The presence of some of the marker chromosomes was also identified in the radiosensitive xrs 5 cell line derived from CHO K1. These results indicate instability of CHO K1 chromosomes containing interstitial telomeric sequences originating from Chinese hamster chromosome 10.

Original language	English
Pages (from-to)	58-60
Number of pages	3
Journal	Cytogenetics and Cell Genetics
Volume	76
Publication status	Published - 1997

Keywords

AMPLIFICATION
SITES
CELLS

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title = "Instability of CHO chromosomes containing interstial telomeric sequences originating from the Chinese hamster chromosome 10",

abstract = "We identified a marker chromosome in the CHO K1 cell line containing amplifications of interstitial telomeric sequences originating from Chinese hamster chromosome 10. Analysis of the progression of this chromosome in two subclones of CHO K1 revealed sensitivity of one amplicon to chromosome breakage, resulting in telomere function at the break site. In addition, two more marker chromosomes, both containing amplifications of interstitial telomeric sequences from chromosome 10, were formed during karyotypic evolution of the CHO K1 subclones. The presence of some of the marker chromosomes was also identified in the radiosensitive xrs 5 cell line derived from CHO K1. These results indicate instability of CHO K1 chromosomes containing interstitial telomeric sequences originating from Chinese hamster chromosome 10.",

keywords = "AMPLIFICATION, SITES, CELLS",

author = "P Slijepcevic and Y Xiao and AT Natarajan and Bryant, {Peter Edward}",

year = "1997",

language = "English",

volume = "76",

pages = "58--60",

journal = "Cytogenetics and Cell Genetics",

issn = "0737-8262",

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TY - JOUR

T1 - Instability of CHO chromosomes containing interstial telomeric sequences originating from the Chinese hamster chromosome 10

AU - Slijepcevic, P

AU - Xiao, Y

AU - Natarajan, AT

AU - Bryant, Peter Edward

PY - 1997

Y1 - 1997

N2 - We identified a marker chromosome in the CHO K1 cell line containing amplifications of interstitial telomeric sequences originating from Chinese hamster chromosome 10. Analysis of the progression of this chromosome in two subclones of CHO K1 revealed sensitivity of one amplicon to chromosome breakage, resulting in telomere function at the break site. In addition, two more marker chromosomes, both containing amplifications of interstitial telomeric sequences from chromosome 10, were formed during karyotypic evolution of the CHO K1 subclones. The presence of some of the marker chromosomes was also identified in the radiosensitive xrs 5 cell line derived from CHO K1. These results indicate instability of CHO K1 chromosomes containing interstitial telomeric sequences originating from Chinese hamster chromosome 10.

AB - We identified a marker chromosome in the CHO K1 cell line containing amplifications of interstitial telomeric sequences originating from Chinese hamster chromosome 10. Analysis of the progression of this chromosome in two subclones of CHO K1 revealed sensitivity of one amplicon to chromosome breakage, resulting in telomere function at the break site. In addition, two more marker chromosomes, both containing amplifications of interstitial telomeric sequences from chromosome 10, were formed during karyotypic evolution of the CHO K1 subclones. The presence of some of the marker chromosomes was also identified in the radiosensitive xrs 5 cell line derived from CHO K1. These results indicate instability of CHO K1 chromosomes containing interstitial telomeric sequences originating from Chinese hamster chromosome 10.

KW - AMPLIFICATION

KW - SITES

KW - CELLS

M3 - Article

SN - 0737-8262

VL - 76

SP - 58

EP - 60

JO - Cytogenetics and Cell Genetics

JF - Cytogenetics and Cell Genetics

ER -

Instability of CHO chromosomes containing interstial telomeric sequences originating from the Chinese hamster chromosome 10

Abstract

Keywords

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