Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species

Andrew G Hettle; Joanne K Hobbs; Benjamin Pluvinage; Chelsea Vickers; Kento T Abe; Orly Salama-Alber; Bailey E McGuire; Jan-Hendrik Hehemann; Joseph P M Hui; Fabrice Berrue; Arjun Banskota; Junzeng Zhang; Eric M Bottos; Jonathan Van Hamme; Alisdair B Boraston

doi:10.1038/s42003-019-0721-y

Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species

Andrew G Hettle, Joanne K Hobbs, Benjamin Pluvinage, Chelsea Vickers, Kento T Abe, Orly Salama-Alber, Bailey E McGuire, Jan-Hendrik Hehemann, Joseph P M Hui, Fabrice Berrue, Arjun Banskota, Junzeng Zhang, Eric M Bottos, Jonathan Van Hamme, Alisdair B Boraston

School of Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Pseudoalteromonas is a globally distributed marine-associated genus that can be found in a broad range of aquatic environments, including in association with macroalgal surfaces where they may take advantage of these rich sources of polysaccharides. The metabolic systems that confer the ability to metabolize this abundant form of photosynthetically fixed carbon, however, are not yet fully understood. Through genomics, transcriptomics, microbiology, and specific structure-function studies of pathway components we address the capacity of newly isolated marine pseudoalteromonads to metabolize the red algal galactan carrageenan. The results reveal that the κ/ι-carrageenan specific polysaccharide utilization locus (CarPUL) enables isolates possessing this locus the ability to grow on this substrate. Biochemical and structural analysis of the enzymatic components of the CarPUL promoted the development of a detailed model of the κ/ι-carrageenan metabolic pathway deployed by pseudoalteromonads, thus furthering our understanding of how these microbes have adapted to a unique environmental niche.

Original language	English
Pages (from-to)	474
Journal	Communications Biology
Volume	2
DOIs	https://doi.org/10.1038/s42003-019-0721-y
Publication status	Published - 2019

Keywords

Aquatic Organisms/metabolism
Binding Sites
Carrageenan/chemistry
Gene Order
Glycoside Hydrolases/chemistry
Metabolic Networks and Pathways
Models, Molecular
Open Reading Frames
Protein Binding
Pseudoalteromonas/genetics
Structure-Activity Relationship

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/s42003-019-0721-y

Cite this

Hettle, A. G., Hobbs, J. K., Pluvinage, B., Vickers, C., Abe, K. T., Salama-Alber, O., McGuire, B. E., Hehemann, J.-H., Hui, J. P. M., Berrue, F., Banskota, A., Zhang, J., Bottos, E. M., Van Hamme, J., & Boraston, A. B. (2019). Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species. Communications Biology, 2, 474. https://doi.org/10.1038/s42003-019-0721-y

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title = "Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species",

abstract = "Pseudoalteromonas is a globally distributed marine-associated genus that can be found in a broad range of aquatic environments, including in association with macroalgal surfaces where they may take advantage of these rich sources of polysaccharides. The metabolic systems that confer the ability to metabolize this abundant form of photosynthetically fixed carbon, however, are not yet fully understood. Through genomics, transcriptomics, microbiology, and specific structure-function studies of pathway components we address the capacity of newly isolated marine pseudoalteromonads to metabolize the red algal galactan carrageenan. The results reveal that the κ/ι-carrageenan specific polysaccharide utilization locus (CarPUL) enables isolates possessing this locus the ability to grow on this substrate. Biochemical and structural analysis of the enzymatic components of the CarPUL promoted the development of a detailed model of the κ/ι-carrageenan metabolic pathway deployed by pseudoalteromonads, thus furthering our understanding of how these microbes have adapted to a unique environmental niche.",

keywords = "Aquatic Organisms/metabolism, Binding Sites, Carrageenan/chemistry, Gene Order, Glycoside Hydrolases/chemistry, Metabolic Networks and Pathways, Models, Molecular, Open Reading Frames, Protein Binding, Pseudoalteromonas/genetics, Structure-Activity Relationship",

author = "Hettle, {Andrew G} and Hobbs, {Joanne K} and Benjamin Pluvinage and Chelsea Vickers and Abe, {Kento T} and Orly Salama-Alber and McGuire, {Bailey E} and Jan-Hendrik Hehemann and Hui, {Joseph P M} and Fabrice Berrue and Arjun Banskota and Junzeng Zhang and Bottos, {Eric M} and {Van Hamme}, Jonathan and Boraston, {Alisdair B}",

note = "{\textcopyright} The Author(s) 2019.",

year = "2019",

doi = "10.1038/s42003-019-0721-y",

language = "English",

volume = "2",

pages = "474",

journal = "Communications Biology",

issn = "2399-3642",

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Hettle, AG, Hobbs, JK, Pluvinage, B, Vickers, C, Abe, KT, Salama-Alber, O, McGuire, BE, Hehemann, J-H, Hui, JPM, Berrue, F, Banskota, A, Zhang, J, Bottos, EM, Van Hamme, J & Boraston, AB 2019, 'Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species', Communications Biology, vol. 2, pp. 474. https://doi.org/10.1038/s42003-019-0721-y

TY - JOUR

T1 - Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species

AU - Hettle, Andrew G

AU - Hobbs, Joanne K

AU - Pluvinage, Benjamin

AU - Vickers, Chelsea

AU - Abe, Kento T

AU - Salama-Alber, Orly

AU - McGuire, Bailey E

AU - Hehemann, Jan-Hendrik

AU - Hui, Joseph P M

AU - Berrue, Fabrice

AU - Banskota, Arjun

AU - Zhang, Junzeng

AU - Bottos, Eric M

AU - Van Hamme, Jonathan

AU - Boraston, Alisdair B

N1 - © The Author(s) 2019.

PY - 2019

Y1 - 2019

N2 - Pseudoalteromonas is a globally distributed marine-associated genus that can be found in a broad range of aquatic environments, including in association with macroalgal surfaces where they may take advantage of these rich sources of polysaccharides. The metabolic systems that confer the ability to metabolize this abundant form of photosynthetically fixed carbon, however, are not yet fully understood. Through genomics, transcriptomics, microbiology, and specific structure-function studies of pathway components we address the capacity of newly isolated marine pseudoalteromonads to metabolize the red algal galactan carrageenan. The results reveal that the κ/ι-carrageenan specific polysaccharide utilization locus (CarPUL) enables isolates possessing this locus the ability to grow on this substrate. Biochemical and structural analysis of the enzymatic components of the CarPUL promoted the development of a detailed model of the κ/ι-carrageenan metabolic pathway deployed by pseudoalteromonads, thus furthering our understanding of how these microbes have adapted to a unique environmental niche.

AB - Pseudoalteromonas is a globally distributed marine-associated genus that can be found in a broad range of aquatic environments, including in association with macroalgal surfaces where they may take advantage of these rich sources of polysaccharides. The metabolic systems that confer the ability to metabolize this abundant form of photosynthetically fixed carbon, however, are not yet fully understood. Through genomics, transcriptomics, microbiology, and specific structure-function studies of pathway components we address the capacity of newly isolated marine pseudoalteromonads to metabolize the red algal galactan carrageenan. The results reveal that the κ/ι-carrageenan specific polysaccharide utilization locus (CarPUL) enables isolates possessing this locus the ability to grow on this substrate. Biochemical and structural analysis of the enzymatic components of the CarPUL promoted the development of a detailed model of the κ/ι-carrageenan metabolic pathway deployed by pseudoalteromonads, thus furthering our understanding of how these microbes have adapted to a unique environmental niche.

KW - Aquatic Organisms/metabolism

KW - Binding Sites

KW - Carrageenan/chemistry

KW - Gene Order

KW - Glycoside Hydrolases/chemistry

KW - Metabolic Networks and Pathways

KW - Models, Molecular

KW - Open Reading Frames

KW - Protein Binding

KW - Pseudoalteromonas/genetics

KW - Structure-Activity Relationship

U2 - 10.1038/s42003-019-0721-y

DO - 10.1038/s42003-019-0721-y

M3 - Article

C2 - 31886414

SN - 2399-3642

VL - 2

SP - 474

JO - Communications Biology

JF - Communications Biology

ER -

Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species

Abstract

Keywords

UN SDGs

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Crystal structure of native DauA