TY - JOUR
T1 - Inititiating a structural study of 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli
AU - Buchanan, LV
AU - Mehta, N
AU - Pocivavsek, S
AU - Niranjanakumari, S
AU - Toone, EJ
AU - Naismith, James Henderson
PY - 1999/11
Y1 - 1999/11
N2 - 2-Keto-3-deoxy-6-phosphogluconate aldolase (KDPG aldolase, E.C. 4.1.2.14) is a member of the pyruvate/phosphoenolpyruvate aldolase family. It is also a synthetically useful enzyme, capable of catalyzing the stereoselective aldol addition of pyruvate to a range of unnatural electrophilic substrates. The recombinant protein was purified by a two-step HPLC protocol involving anion-exchange and hydrophobic chromatography. Dynamic light-scattering experiments indicated the protein to be monodisperse. Crystals were obtained using the sitting-drop vapour-diffusion method, with PEG 6K as precipitant. Diffraction data were collected on a frozen crystal to a resolution of 2.26 Angstrom on station PX9.6 at the Daresbury synchrotron. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 53.2, b = 77.9, c = 146.8 Angstrom.
AB - 2-Keto-3-deoxy-6-phosphogluconate aldolase (KDPG aldolase, E.C. 4.1.2.14) is a member of the pyruvate/phosphoenolpyruvate aldolase family. It is also a synthetically useful enzyme, capable of catalyzing the stereoselective aldol addition of pyruvate to a range of unnatural electrophilic substrates. The recombinant protein was purified by a two-step HPLC protocol involving anion-exchange and hydrophobic chromatography. Dynamic light-scattering experiments indicated the protein to be monodisperse. Crystals were obtained using the sitting-drop vapour-diffusion method, with PEG 6K as precipitant. Diffraction data were collected on a frozen crystal to a resolution of 2.26 Angstrom on station PX9.6 at the Daresbury synchrotron. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 53.2, b = 77.9, c = 146.8 Angstrom.
KW - C BOND FORMATION
KW - RESOLUTION
UR - http://www.scopus.com/inward/record.url?scp=0033230496&partnerID=8YFLogxK
M3 - Article
SN - 0907-4449
VL - D55
SP - 1946
EP - 1948
JO - Acta Crystallographica. Section D, Biological crystallography
JF - Acta Crystallographica. Section D, Biological crystallography
ER -