Infectivity studies of Influenza Virus Hemagglutinin Receptor Binding-site Mutants in Mice

J Meisner; KJ Szretter; KC Bradley; WA Langley; ZN Li; BJ Lee; S Theonnes; J Martin; JJ Skehel; Rupert James Martin Russell; JM Katz; DA Steinhauer

doi:10.1128/JVI.01958-07

Infectivity studies of Influenza Virus Hemagglutinin Receptor Binding-site Mutants in Mice

J Meisner, KJ Szretter, KC Bradley, WA Langley, ZN Li, BJ Lee, S Theonnes, J Martin, JJ Skehel, Rupert James Martin Russell, JM Katz, DA Steinhauer

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

The replicative properties of influenza virus hemagglutinin (RA) mutants with altered receptor binding characteristics were analyzed following intranasal inoculation of mice. Among the mutants examined was a virus containing a Y98F substitution at a conserved position in the receptor binding site that leads to a 20-fold reduction in binding. This mutant can replicate as well as wild-type (WT) virus in MDCK cells and in embryonated chicken eggs but is highly attenuated in mice, exhibiting titers in lungs more than 1,000-fold lower than those of the WT. The capacity of the Y98F mutant to induce antibody responses and the structural locations of HA reversion mutations are examined.

Original language	English
Pages (from-to)	5079-5083
Number of pages	5
Journal	Journal of Virology
Volume	82
DOIs	https://doi.org/10.1128/JVI.01958-07
Publication status	Published - May 2008

Keywords

AIRWAY EPITHELIAL-CELLS
A VIRUS
MEMBRANE-FUSION
EGG-ADAPTATION
B VIRUSES
SPECIFICITY
REPLICATION
HOST
GALACTOSE
RECOGNITION

Access to Document

10.1128/JVI.01958-07

Cite this

@article{5f050b5e6b0943a39d53189ef18ea85f,

title = "Infectivity studies of Influenza Virus Hemagglutinin Receptor Binding-site Mutants in Mice",

abstract = "The replicative properties of influenza virus hemagglutinin (RA) mutants with altered receptor binding characteristics were analyzed following intranasal inoculation of mice. Among the mutants examined was a virus containing a Y98F substitution at a conserved position in the receptor binding site that leads to a 20-fold reduction in binding. This mutant can replicate as well as wild-type (WT) virus in MDCK cells and in embryonated chicken eggs but is highly attenuated in mice, exhibiting titers in lungs more than 1,000-fold lower than those of the WT. The capacity of the Y98F mutant to induce antibody responses and the structural locations of HA reversion mutations are examined.",

keywords = "AIRWAY EPITHELIAL-CELLS, A VIRUS, MEMBRANE-FUSION, EGG-ADAPTATION, B VIRUSES, SPECIFICITY, REPLICATION, HOST, GALACTOSE, RECOGNITION",

author = "J Meisner and KJ Szretter and KC Bradley and WA Langley and ZN Li and BJ Lee and S Theonnes and J Martin and JJ Skehel and Russell, {Rupert James Martin} and JM Katz and DA Steinhauer",

year = "2008",

month = may,

doi = "10.1128/JVI.01958-07",

language = "English",

volume = "82",

pages = "5079--5083",

journal = "Journal of Virology",

issn = "0022-538X",

publisher = "American Society for Microbiology",

}

TY - JOUR

T1 - Infectivity studies of Influenza Virus Hemagglutinin Receptor Binding-site Mutants in Mice

AU - Meisner, J

AU - Szretter, KJ

AU - Bradley, KC

AU - Langley, WA

AU - Li, ZN

AU - Lee, BJ

AU - Theonnes, S

AU - Martin, J

AU - Skehel, JJ

AU - Russell, Rupert James Martin

AU - Katz, JM

AU - Steinhauer, DA

PY - 2008/5

Y1 - 2008/5

N2 - The replicative properties of influenza virus hemagglutinin (RA) mutants with altered receptor binding characteristics were analyzed following intranasal inoculation of mice. Among the mutants examined was a virus containing a Y98F substitution at a conserved position in the receptor binding site that leads to a 20-fold reduction in binding. This mutant can replicate as well as wild-type (WT) virus in MDCK cells and in embryonated chicken eggs but is highly attenuated in mice, exhibiting titers in lungs more than 1,000-fold lower than those of the WT. The capacity of the Y98F mutant to induce antibody responses and the structural locations of HA reversion mutations are examined.

AB - The replicative properties of influenza virus hemagglutinin (RA) mutants with altered receptor binding characteristics were analyzed following intranasal inoculation of mice. Among the mutants examined was a virus containing a Y98F substitution at a conserved position in the receptor binding site that leads to a 20-fold reduction in binding. This mutant can replicate as well as wild-type (WT) virus in MDCK cells and in embryonated chicken eggs but is highly attenuated in mice, exhibiting titers in lungs more than 1,000-fold lower than those of the WT. The capacity of the Y98F mutant to induce antibody responses and the structural locations of HA reversion mutations are examined.

KW - AIRWAY EPITHELIAL-CELLS

KW - A VIRUS

KW - MEMBRANE-FUSION

KW - EGG-ADAPTATION

KW - B VIRUSES

KW - SPECIFICITY

KW - REPLICATION

KW - HOST

KW - GALACTOSE

KW - RECOGNITION

UR - http://www.scopus.com/inward/record.url?scp=43249115708&partnerID=8YFLogxK

U2 - 10.1128/JVI.01958-07

DO - 10.1128/JVI.01958-07

M3 - Article

SN - 0022-538X

VL - 82

SP - 5079

EP - 5083

JO - Journal of Virology

JF - Journal of Virology

ER -

Infectivity studies of Influenza Virus Hemagglutinin Receptor Binding-site Mutants in Mice

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this