Induction of an interferon response by RNAi vectors in mammalian cells

A Bridge; S Pebernard; A Ducraux; A-L Nicoulaz; Richard Derek Iggo

doi:10.1038/ng1173

Induction of an interferon response by RNAi vectors in mammalian cells

A Bridge, S Pebernard, A Ducraux, A-L Nicoulaz, Richard Derek Iggo

School of Medicine

Research output: Contribution to journal › Article › peer-review

865 Citations (Scopus)

Abstract

DNA vectors that express short hairpin RNAs (shRNAs) from RNA polymerase III (Pol III) promoters are a promising new tool to reduce gene expression in mammalian cells. shRNAs are processed to small interfering RNAs (siRNAs) of 21 nucleotides (nt) that guide the cleavage of the cognate mRNA by the RNA-induced silencing complex. Although siRNAs are thought to be too short to induce interferon expression, we report here that a substantial number of shRNA vectors can trigger an interferon response.

Original language	English
Pages (from-to)	263-264
Number of pages	2
Journal	Nature Genetics
Volume	34
Issue number	3
DOIs	https://doi.org/10.1038/ng1173
Publication status	Published - Jul 2003

Keywords

EXPRESSION
VIRUS
GENE

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10.1038/ng1173

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title = "Induction of an interferon response by RNAi vectors in mammalian cells",

abstract = "DNA vectors that express short hairpin RNAs (shRNAs) from RNA polymerase III (Pol III) promoters are a promising new tool to reduce gene expression in mammalian cells. shRNAs are processed to small interfering RNAs (siRNAs) of 21 nucleotides (nt) that guide the cleavage of the cognate mRNA by the RNA-induced silencing complex. Although siRNAs are thought to be too short to induce interferon expression, we report here that a substantial number of shRNA vectors can trigger an interferon response.",

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AU - Bridge, A

AU - Pebernard, S

AU - Ducraux, A

AU - Nicoulaz, A-L

AU - Iggo, Richard Derek

PY - 2003/7

Y1 - 2003/7

N2 - DNA vectors that express short hairpin RNAs (shRNAs) from RNA polymerase III (Pol III) promoters are a promising new tool to reduce gene expression in mammalian cells. shRNAs are processed to small interfering RNAs (siRNAs) of 21 nucleotides (nt) that guide the cleavage of the cognate mRNA by the RNA-induced silencing complex. Although siRNAs are thought to be too short to induce interferon expression, we report here that a substantial number of shRNA vectors can trigger an interferon response.

AB - DNA vectors that express short hairpin RNAs (shRNAs) from RNA polymerase III (Pol III) promoters are a promising new tool to reduce gene expression in mammalian cells. shRNAs are processed to small interfering RNAs (siRNAs) of 21 nucleotides (nt) that guide the cleavage of the cognate mRNA by the RNA-induced silencing complex. Although siRNAs are thought to be too short to induce interferon expression, we report here that a substantial number of shRNA vectors can trigger an interferon response.

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KW - VIRUS

KW - GENE

U2 - 10.1038/ng1173

DO - 10.1038/ng1173

M3 - Article

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VL - 34

SP - 263

EP - 264

JO - Nature Genetics

JF - Nature Genetics

IS - 3

ER -

Induction of an interferon response by RNAi vectors in mammalian cells

Abstract

Keywords

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