Induction of an interferon response by RNAi vectors in mammalian cells

A Bridge, S Pebernard, A Ducraux, A-L Nicoulaz, Richard Derek Iggo

Research output: Contribution to journalArticlepeer-review

835 Citations (Scopus)


DNA vectors that express short hairpin RNAs (shRNAs) from RNA polymerase III (Pol III) promoters are a promising new tool to reduce gene expression in mammalian cells. shRNAs are processed to small interfering RNAs (siRNAs) of 21 nucleotides (nt) that guide the cleavage of the cognate mRNA by the RNA-induced silencing complex. Although siRNAs are thought to be too short to induce interferon expression, we report here that a substantial number of shRNA vectors can trigger an interferon response.

Original languageEnglish
Pages (from-to)263-264
Number of pages2
JournalNature Genetics
Issue number3
Publication statusPublished - Jul 2003


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