Increased sensitivity of malaria detection by nested polymerase chain reaction using simple sampling and DNA extraction

J  Cox-Singh; S  Mahayet; M S  Abdullah; B  Singh; Janet Cox Singh

Increased sensitivity of malaria detection by nested polymerase chain reaction using simple sampling and DNA extraction

J Cox-Singh, S Mahayet, M S Abdullah, B Singh, Janet Cox Singh

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Malaria remains a disease of underdeveloped and remote regions of the world. The application of polymerase chain reaction (PCR) technology to malaria epidemiology has the potential for increasing our knowledge and understanding of this disease. In order to study malaria in all geographical locations it is important that specimen collection and DNA extraction for PCR be kept simple. Here we report a method for extracting DNA from dried blood spots on filter paper which is capable of detecting one Plasmodium falciparum and two Plasmodium vivax parasites/mu l of whole blood by nested PCR without compromising the simplicity of specimen collection or DNA extraction. (C) 1997 Australian Society for Parasitology. Published by Elsevier Science Ltd.

Original language	English
Pages (from-to)	1575-1577
Number of pages	3
Journal	International Journal for Parasitology
Volume	27
Issue number	12
Publication status	Published - Dec 1997

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This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Increased sensitivity of malaria detection by nested polymerase chain reaction using simple sampling and DNA extraction",

abstract = "Malaria remains a disease of underdeveloped and remote regions of the world. The application of polymerase chain reaction (PCR) technology to malaria epidemiology has the potential for increasing our knowledge and understanding of this disease. In order to study malaria in all geographical locations it is important that specimen collection and DNA extraction for PCR be kept simple. Here we report a method for extracting DNA from dried blood spots on filter paper which is capable of detecting one Plasmodium falciparum and two Plasmodium vivax parasites/mu l of whole blood by nested PCR without compromising the simplicity of specimen collection or DNA extraction. (C) 1997 Australian Society for Parasitology. Published by Elsevier Science Ltd.",

author = "J Cox-Singh and S Mahayet and Abdullah, {M S} and B Singh and {Cox Singh}, Janet",

year = "1997",

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language = "English",

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pages = "1575--1577",

journal = "International Journal for Parasitology",

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T1 - Increased sensitivity of malaria detection by nested polymerase chain reaction using simple sampling and DNA extraction

AU - Cox-Singh, J

AU - Mahayet, S

AU - Abdullah, M S

AU - Singh, B

AU - Cox Singh, Janet

PY - 1997/12

Y1 - 1997/12

N2 - Malaria remains a disease of underdeveloped and remote regions of the world. The application of polymerase chain reaction (PCR) technology to malaria epidemiology has the potential for increasing our knowledge and understanding of this disease. In order to study malaria in all geographical locations it is important that specimen collection and DNA extraction for PCR be kept simple. Here we report a method for extracting DNA from dried blood spots on filter paper which is capable of detecting one Plasmodium falciparum and two Plasmodium vivax parasites/mu l of whole blood by nested PCR without compromising the simplicity of specimen collection or DNA extraction. (C) 1997 Australian Society for Parasitology. Published by Elsevier Science Ltd.

AB - Malaria remains a disease of underdeveloped and remote regions of the world. The application of polymerase chain reaction (PCR) technology to malaria epidemiology has the potential for increasing our knowledge and understanding of this disease. In order to study malaria in all geographical locations it is important that specimen collection and DNA extraction for PCR be kept simple. Here we report a method for extracting DNA from dried blood spots on filter paper which is capable of detecting one Plasmodium falciparum and two Plasmodium vivax parasites/mu l of whole blood by nested PCR without compromising the simplicity of specimen collection or DNA extraction. (C) 1997 Australian Society for Parasitology. Published by Elsevier Science Ltd.

M3 - Article

SN - 0020-7519

VL - 27

SP - 1575

EP - 1577

JO - International Journal for Parasitology

JF - International Journal for Parasitology

IS - 12

ER -

Increased sensitivity of malaria detection by nested polymerase chain reaction using simple sampling and DNA extraction

Abstract

UN SDGs

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