Incorporation of choline into Streptococcus pneumoniae cell wall antigens: Evidence for choline kinase activity

Gail C. Whiting, Stephen H. Gillespie*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

The choline-containing teichoic and lipoteichoic acids play an important part in cell wall metabolism of Streptococcus pneumoniae. We propose that a choline kinase enzyme has a role in the synthesis of these antigens. The presence of this enzyme was demonstrated in cell free extracts of S. pneumoniae by measuring the fall in ATP concentration due to phosphorylation of choline. Genomic DNA of S. pneumoniae hybridised with a probe consisting of an internal fragment of the choline kinase gene of Saccharomyces cerevisiae and one consisting of the choline binding domain of lytA.

Original languageEnglish
Pages (from-to)141-145
Number of pages5
JournalFEMS Microbiology Letters
Volume138
Issue number2-3
DOIs
Publication statusPublished - 1 May 1996

Keywords

  • Choline kinase
  • Choline metabolism
  • Lipoteichoic acid
  • Streptococcus pneumoniae
  • Teichoic acid

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