Incorporation of choline into Streptococcus pneumoniae cell wall antigens: Evidence for choline kinase activity

Gail C. Whiting; Stephen H. Gillespie

doi:10.1016/0378-1097(96)00086-9

Incorporation of choline into Streptococcus pneumoniae cell wall antigens: Evidence for choline kinase activity

Gail C. Whiting, Stephen H. Gillespie^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The choline-containing teichoic and lipoteichoic acids play an important part in cell wall metabolism of Streptococcus pneumoniae. We propose that a choline kinase enzyme has a role in the synthesis of these antigens. The presence of this enzyme was demonstrated in cell free extracts of S. pneumoniae by measuring the fall in ATP concentration due to phosphorylation of choline. Genomic DNA of S. pneumoniae hybridised with a probe consisting of an internal fragment of the choline kinase gene of Saccharomyces cerevisiae and one consisting of the choline binding domain of lytA.

Original language	English
Pages (from-to)	141-145
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	138
Issue number	2-3
DOIs	https://doi.org/10.1016/0378-1097(96)00086-9
Publication status	Published - 1 May 1996

Keywords

Choline kinase
Choline metabolism
Lipoteichoic acid
Streptococcus pneumoniae
Teichoic acid

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10.1016/0378-1097(96)00086-9

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title = "Incorporation of choline into Streptococcus pneumoniae cell wall antigens: Evidence for choline kinase activity",

abstract = "The choline-containing teichoic and lipoteichoic acids play an important part in cell wall metabolism of Streptococcus pneumoniae. We propose that a choline kinase enzyme has a role in the synthesis of these antigens. The presence of this enzyme was demonstrated in cell free extracts of S. pneumoniae by measuring the fall in ATP concentration due to phosphorylation of choline. Genomic DNA of S. pneumoniae hybridised with a probe consisting of an internal fragment of the choline kinase gene of Saccharomyces cerevisiae and one consisting of the choline binding domain of lytA.",

keywords = "Choline kinase, Choline metabolism, Lipoteichoic acid, Streptococcus pneumoniae, Teichoic acid",

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AU - Whiting, Gail C.

AU - Gillespie, Stephen H.

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Y1 - 1996/5/1

N2 - The choline-containing teichoic and lipoteichoic acids play an important part in cell wall metabolism of Streptococcus pneumoniae. We propose that a choline kinase enzyme has a role in the synthesis of these antigens. The presence of this enzyme was demonstrated in cell free extracts of S. pneumoniae by measuring the fall in ATP concentration due to phosphorylation of choline. Genomic DNA of S. pneumoniae hybridised with a probe consisting of an internal fragment of the choline kinase gene of Saccharomyces cerevisiae and one consisting of the choline binding domain of lytA.

AB - The choline-containing teichoic and lipoteichoic acids play an important part in cell wall metabolism of Streptococcus pneumoniae. We propose that a choline kinase enzyme has a role in the synthesis of these antigens. The presence of this enzyme was demonstrated in cell free extracts of S. pneumoniae by measuring the fall in ATP concentration due to phosphorylation of choline. Genomic DNA of S. pneumoniae hybridised with a probe consisting of an internal fragment of the choline kinase gene of Saccharomyces cerevisiae and one consisting of the choline binding domain of lytA.

KW - Choline kinase

KW - Choline metabolism

KW - Lipoteichoic acid

KW - Streptococcus pneumoniae

KW - Teichoic acid

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JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2-3

ER -

Incorporation of choline into Streptococcus pneumoniae cell wall antigens: Evidence for choline kinase activity

Abstract

Keywords

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