Improved Growth Kinetics of Enteric Adenovirus Type 40 in a Modified Cell Line that Can No Longer Respond to Interferon Stimulation

V Sherwoods, H-G Burgert, Y-H Chen, S Shanghera, None Katafigiotis, D F Young, W Fielder, I Connerton, Richard Edward Randall, K Mellits

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Human enteric adenoviruses propagate poorly in conventional human cell lines used to grow other adenovirus serotypes. As human enteric adenoviruses have a defect in counteracting the cellular interferon (IFN) response in cell culture, to aid in growth of the virus, a 293-based cell line defective in its ability to respond to IFN was constructed. This cell line (293-SV5/V) constitutively expresses V-protein of the paramyxovirus Simian virus 5, which degrades the signal transducer and activator of transcription 1 (STAT1) and thereby prevents the STAT1-mediated IFN response. Analysis of human enteric adenovirus type 40 (HAdV-40)-infected 293-SV5/V cells compared with parental 293 cells shows that the recombinant line allows more rapid production of virus and results in higher titres. These results suggest that the defect in HAdV-40 in counteracting the IFN response can be overcome at least partially through the use of 293-SV5/V cell lines.

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalJournal of General Virology
Volume88
DOIs
Publication statusPublished - Jan 2007

Keywords

  • VIRUS-REPLICATION
  • ACTIVATION
  • STAT1
  • GASTROENTERITIS
  • SIMIAN-VIRUS-5
  • DEGRADATION
  • SENSITIVITY
  • CONTRIBUTE
  • PROTEINS
  • PROMOTER

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