TY - CHAP
T1 - Improved diagnosis and treatment monitoring of tuberculosis using stool and the tuberculosis bacterial load assay (TB-MBLA)
AU - Musisi, Emmanuel
AU - Mtafya, Bariki
AU - Wambi, William Saava
AU - Zawedde, Josephine
AU - Sessolo, Abdulwahab
AU - Ssengooba, Willy
AU - Walbaum, Natasha
AU - Ntinginya, Nyanda Elias
AU - Gillespie, Stephen H
AU - Sabiiti, Wilber
PY - 2024
Y1 - 2024
N2 - The diagnosis and monitoring of tuberculosis treatment is difficult as many patients are unable to produce sputum. This means that many patients are treated on the basis of clinical findings and consequently some will be exposed to anti-tuberculosis drugs unnecessarily. Moreover, for those appropriately on treatment and unable to produce a sputum sample, it will be impossible to monitor the response to treatment. We have shown that stool is a potential alternative sample type for diagnosis of tuberculosis. Currently, available protocols like the Xpert MTB/RIF use DNA as a target to detect Mycobacterium tuberculosis in stool but DNA survives long after the organism is dead so it is not certain whether a positive test is from an old or a partially treated infection. The TB MBLA only detects live organisms and thus, can be used to follow the response to treatment. In this chapter, we describe a protocol for TB-MBLA, an RNA-based assay, and apply it to quantify TB bacteria in stool.
AB - The diagnosis and monitoring of tuberculosis treatment is difficult as many patients are unable to produce sputum. This means that many patients are treated on the basis of clinical findings and consequently some will be exposed to anti-tuberculosis drugs unnecessarily. Moreover, for those appropriately on treatment and unable to produce a sputum sample, it will be impossible to monitor the response to treatment. We have shown that stool is a potential alternative sample type for diagnosis of tuberculosis. Currently, available protocols like the Xpert MTB/RIF use DNA as a target to detect Mycobacterium tuberculosis in stool but DNA survives long after the organism is dead so it is not certain whether a positive test is from an old or a partially treated infection. The TB MBLA only detects live organisms and thus, can be used to follow the response to treatment. In this chapter, we describe a protocol for TB-MBLA, an RNA-based assay, and apply it to quantify TB bacteria in stool.
KW - Feces/microbiology
KW - Mycobacterium tuberculosis/isolation & purification
KW - Humans
KW - Bacterial load/methods
KW - Tuberculosis/diagnosis
KW - Antitubercular agents/therapeutic use
KW - DNA, Bacterial/genetics
KW - Sputum/microbiology
UR - https://discover.libraryhub.jisc.ac.uk/search?q=Improved%20diagnosis%20and%20treatment%20monitoring%20of%20tuberculosis%20using%20stool%20and%20the%20tuberculosis%20bacterial%20load%20assay%20%28TB-MBLA%29&rn=1
U2 - 10.1007/978-1-0716-3981-8_15
DO - 10.1007/978-1-0716-3981-8_15
M3 - Chapter
C2 - 38949709
SN - 9781071639801
T3 - Methods in molecular biology
SP - 153
EP - 160
BT - Antibiotic resistance protocols
A2 - Gillespie, Stephen H.
PB - Humana Press
CY - New York
ER -
