Improved detection of higher molecular weight proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on polytetrafluoroethylene surfaces

Catherine Helen Botting

doi:10.1002/rcm.954

Improved detection of higher molecular weight proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on polytetrafluoroethylene surfaces

Catherine Helen Botting

Research output: Contribution to journal › Article › peer-review

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) of proteins was performed on a range of polytetrafluoroethylene (PTFE) surfaces. Sinapinic acid and alpha-cyano-4-hydroxycinnamic acid matrices were compared and the order of application varied to identify the best combination for each surface. It is demonstrated that the use of a PTFE surface improves the intensity of signals obtained for higher molecular weight proteins. Copyright (C) 2003 John Wiley Sons, Ltd.

Original language	English
Pages (from-to)	598-602
Number of pages	5
Journal	Rapid Communications in Mass Spectrometry
Volume	17
Issue number	6
DOIs	https://doi.org/10.1002/rcm.954
Publication status	Published - Mar 2003

Keywords

SAMPLE SUPPORT
GEL-ELECTROPHORESIS
MEMBRANE
PEPTIDES

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10.1002/rcm.954

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title = "Improved detection of higher molecular weight proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on polytetrafluoroethylene surfaces",

abstract = "Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) of proteins was performed on a range of polytetrafluoroethylene (PTFE) surfaces. Sinapinic acid and alpha-cyano-4-hydroxycinnamic acid matrices were compared and the order of application varied to identify the best combination for each surface. It is demonstrated that the use of a PTFE surface improves the intensity of signals obtained for higher molecular weight proteins. Copyright (C) 2003 John Wiley Sons, Ltd.",

keywords = "SAMPLE SUPPORT, GEL-ELECTROPHORESIS, MEMBRANE, PEPTIDES",

author = "Botting, {Catherine Helen}",

note = "The use of a PTFE surface attached to a MALDI target plate is shown to give enhanced signal intensities for higher molecular weight proteins. A number of different matrix preparation methods and forms of PTFE are investigated, in order to determine the most effective method.",

year = "2003",

month = mar,

doi = "10.1002/rcm.954",

language = "English",

volume = "17",

pages = "598--602",

journal = "Rapid Communications in Mass Spectrometry",

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publisher = "John Wiley & Sons, Ltd",

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AU - Botting, Catherine Helen

N1 - The use of a PTFE surface attached to a MALDI target plate is shown to give enhanced signal intensities for higher molecular weight proteins. A number of different matrix preparation methods and forms of PTFE are investigated, in order to determine the most effective method.

PY - 2003/3

Y1 - 2003/3

N2 - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) of proteins was performed on a range of polytetrafluoroethylene (PTFE) surfaces. Sinapinic acid and alpha-cyano-4-hydroxycinnamic acid matrices were compared and the order of application varied to identify the best combination for each surface. It is demonstrated that the use of a PTFE surface improves the intensity of signals obtained for higher molecular weight proteins. Copyright (C) 2003 John Wiley Sons, Ltd.

AB - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) of proteins was performed on a range of polytetrafluoroethylene (PTFE) surfaces. Sinapinic acid and alpha-cyano-4-hydroxycinnamic acid matrices were compared and the order of application varied to identify the best combination for each surface. It is demonstrated that the use of a PTFE surface improves the intensity of signals obtained for higher molecular weight proteins. Copyright (C) 2003 John Wiley Sons, Ltd.

KW - SAMPLE SUPPORT

KW - GEL-ELECTROPHORESIS

KW - MEMBRANE

KW - PEPTIDES

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M3 - Article

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VL - 17

SP - 598

EP - 602

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

IS - 6

ER -

Improved detection of higher molecular weight proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on polytetrafluoroethylene surfaces

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Keywords

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