Improved detection of higher molecular weight proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on polytetrafluoroethylene surfaces

Catherine Helen Botting

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) of proteins was performed on a range of polytetrafluoroethylene (PTFE) surfaces. Sinapinic acid and alpha-cyano-4-hydroxycinnamic acid matrices were compared and the order of application varied to identify the best combination for each surface. It is demonstrated that the use of a PTFE surface improves the intensity of signals obtained for higher molecular weight proteins. Copyright (C) 2003 John Wiley Sons, Ltd.

Original languageEnglish
Pages (from-to)598-602
Number of pages5
JournalRapid Communications in Mass Spectrometry
Volume17
Issue number6
DOIs
Publication statusPublished - Mar 2003

Keywords

  • SAMPLE SUPPORT
  • GEL-ELECTROPHORESIS
  • MEMBRANE
  • PEPTIDES

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