Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry

Alison L Dickson; Eylan Yutuc; Catherine A Thornton; Yuqin Wang; William J Griffiths

doi:10.3389/fendo.2022.1031013

Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry

Alison L Dickson, Eylan Yutuc, Catherine A Thornton, Yuqin Wang, William J Griffiths^*

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human placenta, previously reported in a single publication, and propose a pathway from 22R-hydroxycholesterol to a C₂₇ bile acid of probable structure 3β,20R,22R-trihydroxycholest-5-en-(25R)26-oic acid. The pathway is evident not only in placenta, but pathway intermediates are also found in umbilical cord plasma, maternal plasma and amniotic fluid but not non-pregnant women.

Original language	English
Article number	1031013
Number of pages	19
Journal	Frontiers in Endocrinology
Volume	13
DOIs	https://doi.org/10.3389/fendo.2022.1031013
Publication status	Published - 10 Nov 2022

Keywords

Female
Humans
Pregnancy
Oxysterols
Chromatography, liquid
Mass Spectrometry
Amniotic Fluid/metabolism
Fetal Blood/metabolism
Sterol
CYP11A1
LC-MS
CYP27A1

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10.3389/fendo.2022.1031013Licence: CC BY

Dickson-2022-Identification-of-unusual-FENDO-13-1031013-CCBY
Copyright © 2022 Dickson, Yutuc, Thornton, Wang and Griffiths. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Final published version, 14 MBLicence: CC BY

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title = "Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry",

abstract = "The aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human placenta, previously reported in a single publication, and propose a pathway from 22R-hydroxycholesterol to a C27 bile acid of probable structure 3β,20R,22R-trihydroxycholest-5-en-(25R)26-oic acid. The pathway is evident not only in placenta, but pathway intermediates are also found in umbilical cord plasma, maternal plasma and amniotic fluid but not non-pregnant women.",

keywords = "Female, Humans, Pregnancy, Oxysterols, Chromatography, liquid, Mass Spectrometry, Amniotic Fluid/metabolism, Fetal Blood/metabolism, Sterol, CYP11A1, LC-MS, CYP27A1",

author = "Dickson, {Alison L} and Eylan Yutuc and Thornton, {Catherine A} and Yuqin Wang and Griffiths, {William J}",

note = "Funding: This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC, grant numbers BB/I001735/1, BB/N015932/1 and BB/S019588/1 to WG, BB/L001942/1 to YW), the European Union through European Structural Funds (ESF), as part of the Welsh Government funded Academic Expertise for Business project (to WG and YW). AD was supported via a KESS2 award in association with Markes International from the Welsh Government and the European Social Fund.",

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doi = "10.3389/fendo.2022.1031013",

language = "English",

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journal = "Frontiers in Endocrinology",

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AU - Dickson, Alison L

AU - Yutuc, Eylan

AU - Thornton, Catherine A

AU - Wang, Yuqin

AU - Griffiths, William J

N1 - Funding: This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC, grant numbers BB/I001735/1, BB/N015932/1 and BB/S019588/1 to WG, BB/L001942/1 to YW), the European Union through European Structural Funds (ESF), as part of the Welsh Government funded Academic Expertise for Business project (to WG and YW). AD was supported via a KESS2 award in association with Markes International from the Welsh Government and the European Social Fund.

PY - 2022/11/10

Y1 - 2022/11/10

N2 - The aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human placenta, previously reported in a single publication, and propose a pathway from 22R-hydroxycholesterol to a C27 bile acid of probable structure 3β,20R,22R-trihydroxycholest-5-en-(25R)26-oic acid. The pathway is evident not only in placenta, but pathway intermediates are also found in umbilical cord plasma, maternal plasma and amniotic fluid but not non-pregnant women.

AB - The aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human placenta, previously reported in a single publication, and propose a pathway from 22R-hydroxycholesterol to a C27 bile acid of probable structure 3β,20R,22R-trihydroxycholest-5-en-(25R)26-oic acid. The pathway is evident not only in placenta, but pathway intermediates are also found in umbilical cord plasma, maternal plasma and amniotic fluid but not non-pregnant women.

KW - Female

KW - Humans

KW - Pregnancy

KW - Oxysterols

KW - Chromatography, liquid

KW - Mass Spectrometry

KW - Amniotic Fluid/metabolism

KW - Fetal Blood/metabolism

KW - Sterol

KW - CYP11A1

KW - LC-MS

KW - CYP27A1

U2 - 10.3389/fendo.2022.1031013

DO - 10.3389/fendo.2022.1031013

M3 - Article

C2 - 36440193

SN - 1664-2392

VL - 13

JO - Frontiers in Endocrinology

JF - Frontiers in Endocrinology

M1 - 1031013

ER -

Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry

Abstract

Keywords

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