Identification and characterization of parasitism genes from the pinewood nematode Bursaphelenchus xylophilus reveals a multilayered detoxification strategy

Margarida Espada, Ana Cláudia Silva, Sebastian Eves van den Akker, Peter J. A. Cock, Manuel Mota, John T. Jones*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)
2 Downloads (Pure)

Abstract

The migratory endoparasitic nematode Bursaphelenchus xylophilus, which is the causal agent of pine wilt disease, has phytophagous and mycetophagous phases during its life cycle. This highly unusual feature distinguishes it from other plant-parasitic nematodes and requires profound changes in biology between modes. During the phytophagous stage, the nematode migrates within pine trees, feeding on the contents of parenchymal cells. Like other plant pathogens, B.xylophilus secretes effectors from pharyngeal gland cells into the host during infection. We provide the first description of changes in the morphology of these gland cells between juvenile and adult life stages. Using a comparative transcriptomics approach and an effector identification pipeline, we identify numerous novel parasitism genes which may be important for the mediation of interactions of B.xylophilus with its host. In-depth characterization of all parasitism genes using in situ hybridization reveals two major categories of detoxification proteins, those specifically expressed in either the pharyngeal gland cells or the digestive system. These data suggest that B.xylophilus incorporates effectors in a multilayer detoxification strategy in order to protect itself from host defence responses during phytophagy.

Original languageEnglish
Pages (from-to)286-295
Number of pages10
JournalMolecular Plant Pathology
Volume17
Issue number2
Early online date16 Jun 2015
DOIs
Publication statusPublished - 1 Feb 2016

Keywords

  • Bursaphelenchus xylophilus
  • Effectors
  • Gland cells
  • Transcriptome
  • Xenobiotic metabolism

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