Human interactome of the influenza B virus NS1 protein

Corinna Patzina, Catherine H. Botting, Adolfo García-Sastre, Richard E. Randall, Benjamin G. Hale*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

NS1 proteins of influenza A and B viruses share limited sequence homology, yet both are potent manipulators of host cell processes, particularly interferon (IFN) induction. Although many cellular partners are reported for A/NS1, only a few (e.g. PKR and ISG15) have been identified for B/NS1. Here, affinity-purification and mass spectrometry were used to expand the known host interactome of B/NS1. We identified 22 human proteins as new putative targets for B/NS1, validating several, including DHX9, ILF3, YBX1 and HNRNPC. Consistent with two RNA-binding domains in B/NS1, many of the identified factors bind RNA and some interact with B/NS1 in an RNA-dependent manner. Functional characterization of several B/NS1 interactors identified SNRNP200 as a potential positive regulator of host IFN responses, while ILF3 exhibited dual roles in both IFN induction and influenza B virus replication. These data provide a resource for future investigations into the mechanisms underpinning host cell modulation by influenza B virus NS1.

Original languageEnglish
Article number000909
Pages (from-to)2267-2273
Number of pages7
JournalJournal of General Virology
Volume98
Issue number9
DOIs
Publication statusPublished - 1 Sept 2017

Keywords

  • Influenza
  • Interferon
  • Proteomics
  • Virulence factor
  • Virus-host interaction

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