Histone H3 lysine 4 methylation marks postreplicative human cytomegalovirus chromatin

Alexandra Nitzsche, Charlotte Steinhäusser, Katrin Mücke, Christina Paulus, Michael Nevels

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

In the nuclei of permissive cells, human cytomegalovirus genomes form nucleosomal structures initially resembling heterochromatin but gradually switching to a euchromatin-like state. This switch is characterized by a decrease in histone H3 K9 methylation and a marked increase in H3 tail acetylation and H3 K4 methylation across the viral genome. We used ganciclovir and a mutant virus encoding a reversibly destabilized DNA polymerase to examine the impact of DNA replication on histone modification dynamics at the viral chromatin. The changes in H3 tail acetylation and H3 K9 methylation proceeded in a DNA replication-independent fashion. In contrast, the increase in H3 K4 methylation proved to depend widely on viral DNA synthesis. Consistently, labeling of nascent DNA using "click chemistry" revealed preferential incorporation of methylated H3 K4 into viral (but not cellular) chromatin during or following DNA replication. This study demonstrates largely selective epigenetic tagging of postreplicative human cytomegalovirus chromatin.
Original languageEnglish
Pages (from-to)9817-27
Number of pages11
JournalJournal of Virology
Volume86
Issue number18
DOIs
Publication statusPublished - Sept 2012

Keywords

  • Base Sequence
  • Cell Line
  • Chromatin
  • Cytomegalovirus
  • DNA Replication
  • DNA, Viral
  • Epigenesis, Genetic
  • Euchromatin
  • Genome, Viral
  • Heterochromatin
  • Histones
  • Humans
  • Lysine
  • Methylation
  • Virus Replication

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