Histone H2A/H2B dimer exchange by ATP-dependent chromatin remodeling activities

M Bruno, A Flaus, C Stockdale, C Rencurel, H Ferreira, T Owen-Hughes*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

152 Citations (Scopus)

Abstract

ATP-dependent chromatin remodeling activities function to manipulate chromatin structure during gene regulation. One of the ways in which they do this is by altering the positions of nucleosomes along DNA. Here we provide support for the ability of these complexes to move nucleosomes into positions in which DNA is unraveled from one edge. This is expected to result in the loss of histone-DNA contacts that are important for retention of one H2A/H2B dimer within the nucleosome. Consistent with this we find that several chromatin remodeling complexes are capable of catalyzing the exchange of H2A/H2B dimers between chromatin fragments in an ATP-dependent reaction. This provides eukaryotes with additional means by which they may manipulate chromatin structure.

Original languageEnglish
Pages (from-to)1599-1606
Number of pages8
JournalMolecular Cell
Volume12
Issue number6
Publication statusPublished - Dec 2003

Keywords

  • YEAST SWI/SNF COMPLEX
  • BASE-PAIR RESOLUTION
  • IN-VIVO
  • SACCHAROMYCES-CEREVISIAE
  • NUCLEOSOMAL DNA
  • TRANSCRIPTION
  • MUTATIONS
  • PROMOTER
  • DOMAINS
  • DISRUPT

Fingerprint

Dive into the research topics of 'Histone H2A/H2B dimer exchange by ATP-dependent chromatin remodeling activities'. Together they form a unique fingerprint.

Cite this