Highly sensitive reporter cell line for detection of interferon types I–III and their neutralization by antibodies

Kevin Groen; Roger Kuratli; Lauren Sar; Andri Vasou; Michael Huber; David J. Hughes; Benjamin G. Hale

doi:10.1002/eji.202451325

Highly sensitive reporter cell line for detection of interferon types I–III and their neutralization by antibodies

Kevin Groen, Roger Kuratli, Lauren Sar, Andri Vasou, Michael Huber, David J. Hughes, Benjamin G. Hale^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Interferons (IFNs) are a critical component of innate immune defenses and limit viral disease severity. To advance studies on IFNs and their neutralization by pathogenic autoantibodies, we generated a Renilla luciferase-based reporter cell line capable of detecting the activities of IFN-Is, IFN-II, and IFN-IIIs. The reporter cell line exhibits a 125- to 2000-fold higher sensitivity to IFNs than a commonly used alternative biological reporter system and allows for a rapid and simple live-cell workflow for detecting low titer amounts of neutralizing anti-IFN antibodies.

Original language	English
Article number	2451325
Number of pages	4
Journal	European Journal of Immunology
Volume	Early View
Early online date	4 Oct 2024
DOIs	https://doi.org/10.1002/eji.202451325
Publication status	E-pub ahead of print - 4 Oct 2024

Keywords

Innate immunity
Reporter cells
Luciferase
Autoantibodies
Interferon

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10.1002/eji.202451325Licence: CC BY

Groen-2024-Highly-senstiive-reporter-EJI-2451325-CCBY
Copyright © 2024 The Author(s). European Journal of Immunology published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Final published version, 1.21 MBLicence: CC BY

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@article{ec50734836f74f79809e16cb17ecc5cd,

title = "Highly sensitive reporter cell line for detection of interferon types I–III and their neutralization by antibodies",

abstract = "Interferons (IFNs) are a critical component of innate immune defenses and limit viral disease severity. To advance studies on IFNs and their neutralization by pathogenic autoantibodies, we generated a Renilla luciferase-based reporter cell line capable of detecting the activities of IFN-Is, IFN-II, and IFN-IIIs. The reporter cell line exhibits a 125- to 2000-fold higher sensitivity to IFNs than a commonly used alternative biological reporter system and allows for a rapid and simple live-cell workflow for detecting low titer amounts of neutralizing anti-IFN antibodies.",

keywords = "Innate immunity, Reporter cells, Luciferase, Autoantibodies, Interferon",

author = "Kevin Groen and Roger Kuratli and Lauren Sar and Andri Vasou and Michael Huber and Hughes, \{David J.\} and Hale, \{Benjamin G.\}",

note = "Funding: Financial support for this study was provided by the Academy of Medical Sciences (grant no. SBF003/1028 to DJH), the Wellcome Trust Institutional Strategic Support Fund (to DJH), and the Novartis Foundation for Medical-Biological Research (grant no. 23A069 to BGH). Open access funding provided by Universitat Zurich.",

year = "2024",

month = oct,

day = "4",

doi = "10.1002/eji.202451325",

language = "English",

volume = "Early View",

journal = "European Journal of Immunology",

issn = "0014-2980",

publisher = "Wiley-VCH, Weinheim",

}

TY - JOUR

T1 - Highly sensitive reporter cell line for detection of interferon types I–III and their neutralization by antibodies

AU - Groen, Kevin

AU - Kuratli, Roger

AU - Sar, Lauren

AU - Vasou, Andri

AU - Huber, Michael

AU - Hughes, David J.

AU - Hale, Benjamin G.

N1 - Funding: Financial support for this study was provided by the Academy of Medical Sciences (grant no. SBF003/1028 to DJH), the Wellcome Trust Institutional Strategic Support Fund (to DJH), and the Novartis Foundation for Medical-Biological Research (grant no. 23A069 to BGH). Open access funding provided by Universitat Zurich.

PY - 2024/10/4

Y1 - 2024/10/4

N2 - Interferons (IFNs) are a critical component of innate immune defenses and limit viral disease severity. To advance studies on IFNs and their neutralization by pathogenic autoantibodies, we generated a Renilla luciferase-based reporter cell line capable of detecting the activities of IFN-Is, IFN-II, and IFN-IIIs. The reporter cell line exhibits a 125- to 2000-fold higher sensitivity to IFNs than a commonly used alternative biological reporter system and allows for a rapid and simple live-cell workflow for detecting low titer amounts of neutralizing anti-IFN antibodies.

AB - Interferons (IFNs) are a critical component of innate immune defenses and limit viral disease severity. To advance studies on IFNs and their neutralization by pathogenic autoantibodies, we generated a Renilla luciferase-based reporter cell line capable of detecting the activities of IFN-Is, IFN-II, and IFN-IIIs. The reporter cell line exhibits a 125- to 2000-fold higher sensitivity to IFNs than a commonly used alternative biological reporter system and allows for a rapid and simple live-cell workflow for detecting low titer amounts of neutralizing anti-IFN antibodies.

KW - Innate immunity

KW - Reporter cells

KW - Luciferase

KW - Autoantibodies

KW - Interferon

UR - https://www.scopus.com/pages/publications/85205663118

U2 - 10.1002/eji.202451325

DO - 10.1002/eji.202451325

M3 - Article

SN - 0014-2980

VL - Early View

JO - European Journal of Immunology

JF - European Journal of Immunology

M1 - 2451325

ER -

Highly sensitive reporter cell line for detection of interferon types I–III and their neutralization by antibodies

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ISSF Round 7 - David Hughes: ISSF Round 7 - David Hughes

Dave Hughes ISSF3 round 6: Dave Hughes ISSF3 round 6

Posttranslational control: Posttranslational control of our innate immune response: exploring a novel role for ISGylation

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Highly sensitive reporter cell line for detection of interferon types I–III and their neutralization by antibodies

Abstract

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Projects

ISSF Round 7 - David Hughes: ISSF Round 7 - David Hughes

Dave Hughes ISSF3 round 6: Dave Hughes ISSF3 round 6

Posttranslational control: Posttranslational control of our innate immune response: exploring a novel role for ISGylation

Cite this