High Performance Liquid-Chromatography Separation of Natural and Synthetic Desulfoglucosinolates and Their Chemical Validation by Spectroscopic, NMR and CI-MS Methods

Methods are described for the optimised extraction, desulphation and HPLC separation of desulphoglucosinolates, These methods provide rapid separation, identification and quantitative measurements of glucosinolates extracted from Brassica napus L and related crops, of unusual glucosinolates found in crucifer weed species, and also of synthetic alkylglucosinolates, The desulphoglucosinolates used in these studies were either chemically synthesised (at least one example from each major structural class), or purified from various plant sources. Validation of the identities of the desulphoglucosinolates was by comparison of retention times with standards, and by UV,H-1- and C-13-NMR and chemical ionisation MS analysis. A list of useful species, and the specific tissues, from which high concentrations of standards can be extracted is included. Copyright (C) 2001 John Wiley & Sons, Ltd.