High centrifugation speed improves recovery of M. tuberculosis and yield of culture

Godlove T. Chaula; Lucy Namkinga; Ally Mahadhy; Wilber Sabiiti; Nyanda Elias Ntinginya; Barika Mtafya

doi:10.1016/j.tube.2025.102633

High centrifugation speed improves recovery of M. tuberculosis and yield of culture

Godlove T. Chaula^*, Lucy Namkinga, Ally Mahadhy, Wilber Sabiiti, Nyanda Elias Ntinginya, Barika Mtafya

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Background: We assessed the impact of centrifugation on recovery of Mycobacterium tuberculosis (M.tb).

Methods: We used 0.5 McFarland from the 2 weeks M. tb, H37Rv culture and homogenized sputum for our experiments. Samples were decontaminated by 2 % NaOH for 20 min and with PBS for controls. Decontaminated aliquots were centrifuged at 2000×g, 3000×g and 6000×g for 40 min and inoculated on MGIT and LJ media. MGITs were incubated into the BACTEC MGIT 960 Systems following BD manuals and data analyzed on GraphPad Software.

Results: The positivity (days) for M. tb, H37Rv in MGIT and LJ decreased from 20.4 to 17.7 and from 47.6 to 26.6 at 2000×g and 6000×g, respectively; P >0.05. For controls, MGIT and LJ positivity (days) decreased from 19 to 10 and from 39.2 to 11.2 at 2000×g and 6000×g, respectively; P >0.05. MGIT positivity was 6(60 %) at 2000×g and 8(80 %) at 6000×g, corresponding to mean (±SD) of 13.7 ±6.7 and 9.06 ±4.6 days, respectively for sputum. LJ positivity was 1(10 %) at 2000×g and 7(70 %) at 6000×g. MGIT contamination for controls (sputum) was over 50 % and 80 % for LJ.

Conclusion: Higher centrifugation speed improves yield and sensitivity of TB culture.

Original language	English
Article number	102633
Number of pages	6
Journal	Tuberculosis
Volume	152
Early online date	15 Mar 2025
DOIs	https://doi.org/10.1016/j.tube.2025.102633
Publication status	E-pub ahead of print - 15 Mar 2025

Keywords

Tuberculosis
Diagnostics
Centrifugation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.tube.2025.102633Licence: CC BY

Chaula_2025_Tuberculosis_High-centrifugation-speed-improves_CCBY
Copyright © 2025 The Authors. This is an open access article distributed under the terms of the Creative Commons CC-BY license (https://creativecommons.org/licenses/by/4.0/deed.en), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Final published version, 2.25 MBLicence: CC BY

Cite this

@article{8863c52327b540499bf9ffc4df86f233,

title = "High centrifugation speed improves recovery of M. tuberculosis and yield of culture",

abstract = "Background: We assessed the impact of centrifugation on recovery of Mycobacterium tuberculosis (M.tb).Methods: We used 0.5 McFarland from the 2 weeks M. tb, H37Rv culture and homogenized sputum for our experiments. Samples were decontaminated by 2 % NaOH for 20 min and with PBS for controls. Decontaminated aliquots were centrifuged at 2000×g, 3000×g and 6000×g for 40 min and inoculated on MGIT and LJ media. MGITs were incubated into the BACTEC MGIT 960 Systems following BD manuals and data analyzed on GraphPad Software.Results: The positivity (days) for M. tb, H37Rv in MGIT and LJ decreased from 20.4 to 17.7 and from 47.6 to 26.6 at 2000×g and 6000×g, respectively; P >0.05. For controls, MGIT and LJ positivity (days) decreased from 19 to 10 and from 39.2 to 11.2 at 2000×g and 6000×g, respectively; P >0.05. MGIT positivity was 6(60 %) at 2000×g and 8(80 %) at 6000×g, corresponding to mean (±SD) of 13.7 ±6.7 and 9.06 ±4.6 days, respectively for sputum. LJ positivity was 1(10 %) at 2000×g and 7(70 %) at 6000×g. MGIT contamination for controls (sputum) was over 50 % and 80 % for LJ.Conclusion: Higher centrifugation speed improves yield and sensitivity of TB culture.",

keywords = "Tuberculosis, Diagnostics, Centrifugation",

author = "Chaula, {Godlove T.} and Lucy Namkinga and Ally Mahadhy and Wilber Sabiiti and Ntinginya, {Nyanda Elias} and Barika Mtafya",

note = "Funding: The study was supported by the European and Developing Countries Clinical Trials Partnership (EDCTP) through PanACEA consortium as part of MSc thesis for Mr. Godlove Chaula at the University of Dar es Salaam.",

year = "2025",

month = mar,

day = "15",

doi = "10.1016/j.tube.2025.102633",

language = "English",

volume = "152",

journal = "Tuberculosis",

issn = "1472-9792",

publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - High centrifugation speed improves recovery of M. tuberculosis and yield of culture

AU - Chaula, Godlove T.

AU - Namkinga, Lucy

AU - Mahadhy, Ally

AU - Sabiiti, Wilber

AU - Ntinginya, Nyanda Elias

AU - Mtafya, Barika

N1 - Funding: The study was supported by the European and Developing Countries Clinical Trials Partnership (EDCTP) through PanACEA consortium as part of MSc thesis for Mr. Godlove Chaula at the University of Dar es Salaam.

PY - 2025/3/15

Y1 - 2025/3/15

N2 - Background: We assessed the impact of centrifugation on recovery of Mycobacterium tuberculosis (M.tb).Methods: We used 0.5 McFarland from the 2 weeks M. tb, H37Rv culture and homogenized sputum for our experiments. Samples were decontaminated by 2 % NaOH for 20 min and with PBS for controls. Decontaminated aliquots were centrifuged at 2000×g, 3000×g and 6000×g for 40 min and inoculated on MGIT and LJ media. MGITs were incubated into the BACTEC MGIT 960 Systems following BD manuals and data analyzed on GraphPad Software.Results: The positivity (days) for M. tb, H37Rv in MGIT and LJ decreased from 20.4 to 17.7 and from 47.6 to 26.6 at 2000×g and 6000×g, respectively; P >0.05. For controls, MGIT and LJ positivity (days) decreased from 19 to 10 and from 39.2 to 11.2 at 2000×g and 6000×g, respectively; P >0.05. MGIT positivity was 6(60 %) at 2000×g and 8(80 %) at 6000×g, corresponding to mean (±SD) of 13.7 ±6.7 and 9.06 ±4.6 days, respectively for sputum. LJ positivity was 1(10 %) at 2000×g and 7(70 %) at 6000×g. MGIT contamination for controls (sputum) was over 50 % and 80 % for LJ.Conclusion: Higher centrifugation speed improves yield and sensitivity of TB culture.

AB - Background: We assessed the impact of centrifugation on recovery of Mycobacterium tuberculosis (M.tb).Methods: We used 0.5 McFarland from the 2 weeks M. tb, H37Rv culture and homogenized sputum for our experiments. Samples were decontaminated by 2 % NaOH for 20 min and with PBS for controls. Decontaminated aliquots were centrifuged at 2000×g, 3000×g and 6000×g for 40 min and inoculated on MGIT and LJ media. MGITs were incubated into the BACTEC MGIT 960 Systems following BD manuals and data analyzed on GraphPad Software.Results: The positivity (days) for M. tb, H37Rv in MGIT and LJ decreased from 20.4 to 17.7 and from 47.6 to 26.6 at 2000×g and 6000×g, respectively; P >0.05. For controls, MGIT and LJ positivity (days) decreased from 19 to 10 and from 39.2 to 11.2 at 2000×g and 6000×g, respectively; P >0.05. MGIT positivity was 6(60 %) at 2000×g and 8(80 %) at 6000×g, corresponding to mean (±SD) of 13.7 ±6.7 and 9.06 ±4.6 days, respectively for sputum. LJ positivity was 1(10 %) at 2000×g and 7(70 %) at 6000×g. MGIT contamination for controls (sputum) was over 50 % and 80 % for LJ.Conclusion: Higher centrifugation speed improves yield and sensitivity of TB culture.

KW - Tuberculosis

KW - Diagnostics

KW - Centrifugation

U2 - 10.1016/j.tube.2025.102633

DO - 10.1016/j.tube.2025.102633

M3 - Article

SN - 1472-9792

VL - 152

JO - Tuberculosis

JF - Tuberculosis

M1 - 102633

ER -

High centrifugation speed improves recovery of M. tuberculosis and yield of culture

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this